GFP Tag anticorps (N-Term)

N° du produit ABIN2443932

Cet anticorps anti-GFP Tag Monoclonal Souris (Clone GF28R) (ABIN2443932) détecte spécifiquement GFP Tag dans WB, ELISA, IHC, IP et DB. L’anticorps est réactif avec des échantillons de Aequorea victoria.

Aperçu rapide pour GFP Tag anticorps (N-Term) (ABIN2443932)

Antigène: GFP Tag

Reactivité: Aequorea victoria

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFP Tag est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Dot Blot (DB)

Clone: GF28R

Détail du produit anti-GFP Tag anticorps

Épitope: N-Term

Fonction: GFP Tag Mouse Monoclonal Antibody (GF28R)

Specificité: The antibody recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP.

Purification: Protein G purified

Immunogène: GFP from the jellyfish Aequorea victoria N-terminal peptide-KLH conjugates.

Isotype: IgG2b

Information d'application

Indications d'application: WB 1:1000,IHC 1:500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Date de péremption: 24 months

Détails sur GFP Tag

Antigène: GFP Tag

Classe de substances: Tag

Sujet: Epitope tags have application in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. The green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is involved in the bioluminescence of the jellyfish. When expressed in prokaryotic cells, GFP produces a fluorescent product, making GFP useful in monitoring physiological processes, visualizing protein localization, and detecting transgenic expression.

UniProt: P42212