GFP Tag anticorps

N° du produit ABIN387749

Détail du produit anti-GFP Tag anticorps

Antigène: GFP Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFP Tag est non-conjugé

Application: Western Blotting (WB)

Purification: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Immunogène: Purified His-tagged GFP protein was used to produced this monoclonal antibody.

Clone: 168AT1211-269-64

Isotype: IgG

Information d'application

Indications d'application: WB: 1:100~500. WB: 1:100~500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Mouse monoclonal antibody supplied in crude ascites with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 6 months

Références pour anticorps anti-GFP Tag (ABIN387749)

Netsu, Hiraguri, Uehara-Ichiki, Komatsu, Sasaya: "Functional comparison of RNA silencing suppressor between the p5 protein of rice grassy stunt virus and the p3 protein of rice stripe virus." dans: Virus research, Vol. 203, pp. 10-9, (2015) (PubMed).

Nicholls, Chu, Abbruzzese, Tremblay, Hardy: "Mechanism of a genetically encoded dark-to-bright reporter for caspase activity." dans: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 24977-86, (2011) (PubMed).

Détails sur GFP Tag

Antigène: GFP Tag

Abstract: GFP Tag Produits

Synonymes: anticorps green fluorescent protein, anticorps gfp

Classe de substances: Tag

Sujet: Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.