RAD18 anticorps (C-Term)

N° du produit ABIN2452086

Cet anticorps anti-RAD18 est un anticorps Lapin Polyclonal détectant RAD18 dans WB, ELISA, IF et IP. Adapté pour Humain. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour RAD18 anticorps (C-Term) (ABIN2452086)

Antigène: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD18 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Détail du produit anti-RAD18 anticorps

Épitope: C-Term

Réactivité croisée (Details): Not reactive to mouse Rad18

Purification: Affinity purified with His-tagged Rad18 protein affinity column

Immunogène: GST-fusion protein containing 113 carboxyl terminal of human Rad18

Isotype: IgG

Information d'application

Indications d'application: 1) Western blotting: 1,000 fold dilution
2) Indirect immunofluorescence staining
3) Immunoprecipitation

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Agent conservateur: Azide free

Stock: -20 °C

Références pour anticorps anti-RAD18 (ABIN2452086)

Watanabe, Tateishi, Kawasuji, Tsurimoto, Inoue, Yamaizumi: "Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination." dans: The EMBO journal, Vol. 23, Issue 19, pp. 3886-96, (2004) (PubMed).

Miyase, Tateishi, Watanabe, Tomita, Suzuki, Inoue, Yamaizumi: "Differential regulation of Rad18 through Rad6-dependent mono- and polyubiquitination." dans: The Journal of biological chemistry, Vol. 280, Issue 1, pp. 515-24, (2004) (PubMed).

Tateishi, Sakuraba, Masuyama, Inoue, Yamaizumi: "Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 14, pp. 7927-32, (2000) (PubMed).

Détails sur RAD18

Antigène: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Autre désignation: Rad18

Sujet: The Rad6-Rad18 pair of genes plays a critical role in post-replication repair of damaged DNA. Rad6 protein functions as an E2 enzyme and Rad18 (495 aa, 56 kDa) as an ubiquitine ligase (E3) which ubiquitinates PCNA. Rad18 recruits translesion DNA polymerases to damaged DNA.