PCK1 anticorps
Aperçu rapide pour PCK1 anticorps (ABIN2460581)
Antigène
Voir toutes PCK1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Purification
- Antibody is purified by protein A chromatography method.
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Immunogène
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCK1.
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Indications d'application
- PCK1 antibody can be used for detection of PCK1 by ELISA at 1:1562500. PCK1 antibody can be used for detection of PCK1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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Concentration
- 1 mg/mL
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Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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Conseil sur la manipulation
- As with any antibody avoid repeat freeze-thaw cycles.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store PCK1 antibody at -20 °C.
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- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
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Autre désignation
- PCK1
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Sujet
- PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet.
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Poids moléculaire
- 69 kDa
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ID gène
- 5105
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NCBI Accession
- NP_002582
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UniProt
- P35558
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Pathways
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
Antigène
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