ARID4A anticorps
Aperçu rapide pour ARID4A anticorps (ABIN2460649)
Antigène
Voir toutes ARID4A AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Purification
- Antibody is purified by protein A chromatography method.
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Immunogène
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ARID4A.
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Indications d'application
- ARID4A antibody can be used for detection of ARID4A by ELISA at 1:62500. ARID4A antibody can be used for detection of ARID4A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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Concentration
- 1 mg/mL
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Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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Conseil sur la manipulation
- As with any antibody avoid repeat freeze-thaw cycles.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store ARID4A antibody at -20 °C.
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- ARID4A (AT Rich Interactive Domain 4A (RBP1-Like) (ARID4A))
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Autre désignation
- ARID4A
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Sujet
- ARID4A encodes a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein (pRB) which regulates cell proliferation. pRB represses transcription by recruiting the encoded protein. This protein, in turn, serves as a bridging molecule to recruit HDACs and, in addition, provides a second HDAC-independent repression function. The encoded protein possesses transcriptional repression activity. Multiple alternatively spliced transcripts have been observed for this gene, although not all transcript variants have been fully described.
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Poids moléculaire
- 143 kDa
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ID gène
- 5926
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NCBI Accession
- NP_002883
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UniProt
- P29374
Antigène
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