MCM4 anticorps

N° du produit ABIN2461392

Cet anticorps anti-MCM4 est un anticorps Lapin Polyclonal détectant MCM4 dans WB et ELISA. Adapté pour Humain, Souris, Rat et Chien.

Aperçu rapide pour MCM4 anticorps (ABIN2461392)

Antigène: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MCM4 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-MCM4 anticorps

Purification: Antibody is purified by protein A chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM4.

Information d'application

Indications d'application: MCM4 antibody can be used for detection of MCM4 by ELISA at 1:12500. MCM4 antibody can be used for detection of MCM4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MCM4 antibody at -20 °C.

Détails sur MCM4

Antigène: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Autre désignation: MCM4

Sujet: The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. The MCM4 gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks.

Poids moléculaire: 95 kDa, 97 kDa

ID gène: 4173

NCBI Accession: NP_005905

UniProt: P33991

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA