NONO anticorps

N° du produit ABIN2462238

Détail du produit anti-NONO anticorps

Antigène: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NONO est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by protein A chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NONO.

Information d'application

Indications d'application: NONO antibody can be used for detection of NONO by ELISA at 1:312500. NONO antibody can be used for detection of NONO by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NONO antibody at -20 °C.

Détails sur NONO

Antigène: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Autre désignation: NONO (NONO Produits)

Synonymes: anticorps NMT55, anticorps NRB54, anticorps P54, anticorps P54NRB, anticorps nmt55, anticorps nrb54, anticorps p54nrb, anticorps xp54nrb, anticorps AA407051, anticorps AV149256, anticorps nonA, anticorps non-POU domain containing octamer binding, anticorps non-POU domain containing, octamer-binding, anticorps non-POU domain containing, octamer binding L homeolog, anticorps non-POU-domain-containing, octamer binding protein, anticorps NONO, anticorps Nono, anticorps nono.L

Sujet: NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V (D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.

Poids moléculaire: 52 kDa

ID gène: 4841

NCBI Accession: NP_031389

UniProt: Q15233