IDH3A anticorps

N° du produit ABIN2462553

Cet anticorps anti-IDH3A est un anticorps Polyclonal produit chez le Lapin, détectant IDH3A dans WB, ELISA et IHC. Convient pour Humain, Rat, Souris et Chien.

Aperçu rapide pour IDH3A anticorps (ABIN2462553)

Antigène: IDH3A (Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A))

Reactivité: Humain, Rat, Souris, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IDH3A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-IDH3A anticorps

Purification: Antibody is purified by protein A chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IDH3A.

Information d'application

Indications d'application: IDH3A antibody can be used for detection of IDH3A by ELISA at 1:312500. IDH3A antibody can be used for detection of IDH3A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store IDH3A antibody at -20 °C.

Détails sur IDH3A

Antigène: IDH3A (Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A))

Autre désignation: IDH3A

Sujet: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD (+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. IDH3A is the alpha subunit of one isozyme of NAD (+)-dependent isocitrate dehydrogenase.Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD (+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD (+)-dependent isocitrate dehydrogenase.

Poids moléculaire: 40 kDa

ID gène: 3419

NCBI Accession: NP_005521

UniProt: P50213