DPH1 anticorps
Aperçu rapide pour DPH1 anticorps (ABIN2463486)
Antigène
Voir toutes DPH1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Purification
- Antibody is purified by peptide affinity chromatography method.
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Immunogène
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH1.
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Indications d'application
- DPH1 antibody can be used for detection of DPH1 by ELISA at 1:312500. DPH1 antibody can be used for detection of DPH1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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Concentration
- 1 mg/mL
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Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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Conseil sur la manipulation
- As with any antibody avoid repeat freeze-thaw cycles.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store DPH1 antibody at -20 °C.
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- DPH1 (DPH1 Homolog (DPH1))
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Autre désignation
- DPH1
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Sujet
- Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]). Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).
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Poids moléculaire
- 49 kDa
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ID gène
- 1801
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NCBI Accession
- NP_001374
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UniProt
- Q9BZG8
Antigène
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