TBP anticorps

N° du produit ABIN2463874

Cet anticorps Lapin Polyclonal détecte spécifiquement TBP dans WB et ELISA. Il présente une réactivité envers Humain, Souris, Rat, Poisson zèbre (Danio rerio) et Chien.

Aperçu rapide pour TBP anticorps (ABIN2463874)

Antigène: TBP (TATA Box Binding Protein (TBP))

Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio), Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TBP est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-TBP anticorps

Purification: Antibody is purified by protein A chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TBP.

Information d'application

Indications d'application: TBP antibody can be used for detection of TBP by ELISA at 1:62500. TBP antibody can be used for detection of TBP by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store TBP antibody at -20 °C.

Détails sur TBP

Antigène: TBP (TATA Box Binding Protein (TBP))

Autre désignation: TBP

Sujet: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.

Poids moléculaire: 38 kDa

ID gène: 6908

NCBI Accession: NP_003185

UniProt: P20226

Pathways: Signalisation WNT