MSH2 anticorps

N° du produit ABIN2464085

L’anticorps anti-MSH2 Monoclonal Souris est utilisé pour la détection de MSH2 dans des échantillons de Humain. Il a été validé pour WB, IHC et ELISA.

Aperçu rapide pour MSH2 anticorps (ABIN2464085)

Antigène: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MSH2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 3A2B8C

Détail du produit anti-MSH2 anticorps

Immunogène: Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).

Isotype: IgG1

Information d'application

Indications d'application: Western Blot:Dilution 1:200 - 1:1,000
IHC(P):Dilution 1:200 - 1:1,000
IHC(F):Dilution 1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Stockage

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Stock: -20 °C

Stockage commentaire: MSH2 monoclonal antibody can be stored at -20 °C, stable for one year.

Détails sur MSH2

Antigène: MSH2 (Mismatch Repair Protein 2 (MSH2))

Autre désignation: MSH2

Sujet: MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50 % of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.

ID gène: 4436

UniProt: P43246

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response