DARC anticorps (N-Term)

N° du produit ABIN2465620

Cet anticorps Chèvre Polyclonal détecte spécifiquement DARC dans WB, ELISA, IHC et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour DARC anticorps (N-Term) (ABIN2465620)

Antigène: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DARC est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-DARC anticorps

Épitope: N-Term

Purification: Duffy antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Duffy antibody was raised against a 19 amino acid synthetic peptide near the N-Terminus of Duffy.

Information d'application

Indications d'application: ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 35 kDa band observed in human kidney and human liver lysates (calculated MW of 35.6 kDa according to NP_002027.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney, stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on Huamn lung showing endothelial staining. Recommended concentration, 3 μg/mL. FACS: A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20 °C.

Détails sur DARC

Antigène: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

ID gène: 2532

NCBI Accession: NP_002027

UniProt: Q16570