NOTCH2 anticorps (N-Term)

N° du produit ABIN2470258

Cet anticorps Lapin Polyclonal détecte spécifiquement NOTCH2 dans WB, ELISA, IHC et IF. Il présente une réactivité avec des échantillons de Humain, Chien et Chimpanzé.

Aperçu rapide pour NOTCH2 anticorps (N-Term) (ABIN2470258)

Antigène: NOTCH2 (Notch 2 (NOTCH2))

Reactivité: Humain, Chien, Chimpanzé

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NOTCH2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-NOTCH2 anticorps

Épitope: N-Term

Purification: Sterile Filtration

Immunogène: NOTCH2 antibody was raised against amino acid residues 2396 - 2409 of NOTCH2. A residue of cysteine was added to the amino terminal end to facilitate coupling (Human).

Information d'application

Indications d'application: NOTCH2 antibody can be used in Western Blot starting at 0.5 - 4 μg/mL, and immunohistochemistry starting at 5 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: As with all antibodies avoid freeze/thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: Store NOTCH2 antibody at 4 °C or -20 °C.

Détails sur NOTCH2

Antigène: NOTCH2 (Notch 2 (NOTCH2))

Autre désignation: NOTCH2

Sujet: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Notch functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

ID gène: 4853

UniProt: Q04721

Pathways: Signalisation Notch, Stem Cell Maintenance