DNAJB1 anticorps (FITC)

N° du produit ABIN2481541

Cet anticorps Souris Monoclonal détecte spécifiquement DNAJB1 dans WB, ELISA et IP. Il présente une réactivité avec des échantillons de Saccharomyces cerevisiae.

Aperçu rapide pour DNAJB1 anticorps (FITC) (ABIN2481541)

Antigène: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Reactivité: Saccharomyces cerevisiae

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DNAJB1 est conjugé à/à la FITC

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 1G10-H8

Détail du produit anti-DNAJB1 anticorps (FITC)

Specificité: Detects ~40 kDa. Yeast specific product. Does not cross react with Human, Mouse or Rat.

Réactivité croisée: Saccharomyces cerevisiae

Purification: Protein G Purified

Immunogène: Full length protein yeast HSP40 (YDJ1)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

• WB (1:2000)
• optimal dilutions for assays should be determined by the user.

Commentaires:

0.5 μg/ml of ABIN2481541 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur DNAJB1

Antigène: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Autre désignation: HSP40

Sujet: Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of HSP40, type 1 DNAJ proteins including HDJ2 and yeast YdjI, type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (5, 6). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).

ID gène: 855661

NCBI Accession: NP_014335

UniProt: P25491