HSP70/HSC70 anticorps (FITC)

N° du produit ABIN2481624

Cet anticorps anti-HSP70/HSC70 est un anticorps Souris Monoclonal détectant HSP70/HSC70 dans WB, IP, IHC, ICC et IF. Adapté pour Poulet.

Aperçu rapide pour HSP70/HSC70 anticorps (FITC) (ABIN2481624)

Antigène: HSP70/HSC70 (HSC70-4) (Heat Shock Protein Cognate 4 (HSC70-4))

Reactivité: Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSP70/HSC70 est conjugé à/à la FITC

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: BB70

Détail du produit anti-HSP70/HSC70 anticorps (FITC)

Specificité: Detects ~72 (HSP) and ~73 kDa (HSC).

Réactivité croisée: Beluga, Poulet, Boeuf (Vache), Chien, Drosophila melanogaster, Poisson, Cobaye, Hamster, Humain, Souris, Porc, Lapin, Rat, Saccharomyces cerevisiae, Mouton, Xenopus laevis

Purification: Protein G Purified

Immunogène: Chicken HSP70/HSP90 complex

Isotype: IgG2a

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:200)
• ICC/IF (1:200)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN2481624 was sufficient for detection of HSP70 and HSC70 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur HSP70/HSC70

Antigène: HSP70/HSC70 (HSC70-4) (Heat Shock Protein Cognate 4 (HSC70-4))

Autre désignation: HSP70/HSC70

Sujet: HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.

ID gène: 423504

NCBI Accession: NP_001006686

UniProt: P08106