GRP78 anticorps (Biotin)

N° du produit ABIN2484202

L’anticorps anti-GRP78 Monoclonal Souris est utilisé pour la détection de GRP78 dans des échantillons de Humain. Il a été validé pour WB, ICC et IF.

Aperçu rapide pour GRP78 anticorps (Biotin) (ABIN2484202)

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GRP78 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 1H11-1H7

Détail du produit anti-GRP78 anticorps (Biotin)

Specificité: Detects ~78 kDa.

Réactivité croisée: Boeuf (Vache), Fusarium, Hamster, Humain, Singe, Souris, Lapin, Rat, Xenopus laevis

Purification: Protein G Purified

Immunogène: His-tagged human GRP78

Isotype: IgG2b

Information d'application

Indications d'application:

• WB (1:2000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

0.5 μg/ml of ABIN2484202 was sufficient for detection of Grp78 in 10 μg of rat tissue lysate by ECL immunoblot analysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur GRP78

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Autre désignation: GRP78

Sujet: GRP78 is a ubiquitously expressed, 78- kDa glucose- regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Lou et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).

ID gène: 14828

NCBI Accession: NP_001156906

UniProt: P20029

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling