AQP2 anticorps (C-Term) (PE)

N° du produit ABIN2486382

Cet anticorps Lapin Polyclonal détecte spécifiquement AQP2 dans WB, IHC, IF et ICC. Il présente une réactivité envers Rat.

Aperçu rapide pour AQP2 anticorps (C-Term) (PE) (ABIN2486382)

Antigène: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AQP2 est conjugé à/à la PE

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-AQP2 anticorps (PE)

Épitope: C-Term

Specificité: Detects ~28.8 kDa. May detect larger glycosylated bands ~35-50 kDa.

Réactivité croisée: Humain, Souris, Rat

Purification: Protein A Purified

Immunogène: Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2

Information d'application

Indications d'application:

• WB (1:2000)
• IHC (1:200)
• ICC/IF (1:400)
• optimal dilutions for assays should be determined by the user.

Commentaires:

0.5 μg/ml of ABIN2486382 was sufficient for detection of aquaporin 2 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur AQP2

Antigène: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Autre désignation: Aquaporin 2

Sujet: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell (3). Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI) (4).

ID gène: 25386

NCBI Accession: NP_037041

UniProt: P34080

Pathways: Response to Water Deprivation