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Rab4 anticorps (C-Term) (Biotin)

RAB4A Reactivité: Humain WB, IHC, IF, ICC Hôte: Lapin Polyclonal Biotin
N° du produit ABIN2486639
  • Antigène Voir toutes Rab4 (RAB4A) Anticorps
    Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))
    Épitope
    • 16
    • 15
    • 7
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 68
    • 43
    • 25
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 57
    • 9
    • 2
    Lapin
    Clonalité
    • 50
    • 18
    Polyclonal
    Conjugué
    • 30
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Rab4 est conjugé à/à la Biotin
    Application
    • 50
    • 19
    • 17
    • 16
    • 14
    • 14
    • 14
    • 13
    • 5
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificité
    Detects ~26 kDa.
     Réactivité croisée
    Humain, Souris, Rat
    Purification
    Peptide Affinity Purified
    Immunogène
    C-terminal peptide from human Rab4
    Top Product
    Discover our top product RAB4A Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:150)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    A 1:1000 dilution of ABIN2486639 was sufficient for detection of Rab4 in 10 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))
    Autre désignation
    Rab4 (RAB4A Produits)
    Synonymes
    anticorps rab4a, anticorps MGC52945, anticorps fc93b03, anticorps wu:fc93b03, anticorps RAB4A, anticorps HRES-1/RAB4, anticorps RAB4, anticorps AI848268, anticorps Rab4, anticorps RAB4A, member RAS oncogene family, anticorps RAB4a, member RAS oncogene family, anticorps rab4a, anticorps RAB4A, anticorps Rab4a
    Sujet
    Rab4 is a 25 kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COHO-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins (3). Each Rab shows a characteristic sub-cellular distribution (4). In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin (5). Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol (6).
    ID gène
    5867
    NCBI Accession
    NP_004569
    UniProt
    P20338
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