PCK1 anticorps

N° du produit ABIN249350

L’anticorps Lapin Polyclonal anti-PCK1 a été validé pour WB. Il convient pour détecter PCK1 dans des échantillons de Souris, Zea mays, Green bean et Miscanthus giganteus. Il y a 1 publication disponible.

Aperçu rapide pour PCK1 anticorps (ABIN249350)

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivité: Souris, Zea mays, Green bean, Miscanthus giganteus

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCK1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PCK1 anticorps

Réactivité croisée (Details): No cross-reactivity with: Arabidopsis thaliana

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 73 / 78 kDa

Purification: serum

Immunogène: KLH-conjugated synthetic peptide well conserved in both PEPCK1 and 2 sequences from different plant species including Zea mays Q9SLZ0

Information d'application

Indications d'application: Recommended Dilution: 1 : 1000 with standard ECL (WB) MW 73 | 78 kDa.

Commentaires:

due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 µg is adviced when working with this antibody.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Références pour anticorps anti-PCK1 (ABIN249350)

Aragón, Pascual, González, Escalona, Carvalho, Amancio: "The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles." dans: Plant cell reports, (2013) (PubMed).

Détails sur PCK1

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Autre désignation: PEPCK

Sujet: Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) Is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2. Both genes are nuclear, located on chromosome 4 and 5 respectively (in Arabidopsis). The protein products, PEPCK1 and PEPCK2, are both highly conserved in the model plant, Arabidopsis thaliana.

Poids moléculaire: expected: 73 kDa, apparent: 78 kDa

UniProt: Q9SLZ0

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis