D1 Protein of PSII, (PsbA) (C-Term) anticorps

N° du produit ABIN2559394

Cité dans 7 publications. Cet anticorps anti-D1 Protein of PSII, (PsbA) Polyclonal Lapin (ABIN2559394) détecte spécifiquement D1 Protein of PSII, (PsbA) dans WB, BN PAGE, EM et IF. L’anticorps est réactif avec des échantillons de Arabidopsis thaliana, Chlamydomonas reinhardtii, Zea mays, Synechococcus sp., Orge, Anabaena variabilis, Triticum aestivum, Tomato, Spartina alterniflora, Chlorella vulgaris, Synechocystis, Quercus ilex, Miscanthus giganteus, Nicotiana benthamiana, Nicotiana tabacum, Paulinella chromatophora, Pea, Phaeodactylum tricornutum, Physcomytrium Patens, Picea glauca, Pilosella officinarum, Pinus strobus, Prochlorococcus, Proso millet (Panicum miliaceum), Mirkania micrantha, Red Algae (Cyanidioschyzon), Salicornia bigelovii, Soybean, Spinach, Spirodela polyrhiza, Symbiodinium, Alniaria alnifolia, Syntrichia ruralis, Tetradesmus obliquus, Thalassiosira weisflogii, Triticosecale, Alfalfa, Dianthus caryophyllus, Artemisia annua, Begonia, Cannabis sativa L., Chlorella ohadii, Araliaceae (Panax), Chromera velia, Cladocopium goreaui, Colobanthus quitensis, Coscinodiscus wailesii, Craterostigma, Cytisus cantabricus, Desmodesmus, Arundo, Diatom (Skeletonema), Ditylum brightwellii, Eucalyptus globulus, Fraxinus chinensis subsp. Rhynchophylla, Gongolaria barbata, Halomicronema hongdechloris, Lasallia hispanica, Lindernia, Manihot esculenta, Marchantia polymorpha, Megathyrsus maximus et Microcystis aeruginosa.

Aperçu rapide pour D1 Protein of PSII, (PsbA) (C-Term) anticorps (ABIN2559394)

Antigène: D1 Protein of PSII, (PsbA)

Reactivité: Arabidopsis thaliana, Chlamydomonas reinhardtii, Zea mays, Synechococcus sp., Orge, Anabaena variabilis, Triticum aestivum, Tomato, Spartina alterniflora, Chlorella vulgaris, Synechocystis, Quercus ilex, Miscanthus giganteus, Nicotiana benthamiana, Nicotiana tabacum, Paulinella chromatophora, Pea, Phaeodactylum tricornutum, Physcomytrium Patens, Picea glauca, Pilosella officinarum, Pinus strobus, Prochlorococcus, Proso millet (Panicum miliaceum), Mirkania micrantha, Red Algae (Cyanidioschyzon), Salicornia bigelovii, Soybean, Spinach, Spirodela polyrhiza, Symbiodinium, Alniaria alnifolia, Syntrichia ruralis, Tetradesmus obliquus, Thalassiosira weisflogii, Triticosecale, Alfalfa, Dianthus caryophyllus, Artemisia annua, Begonia, Cannabis sativa L., Chlorella ohadii, Araliaceae (Panax), Chromera velia, Cladocopium goreaui, Colobanthus quitensis, Coscinodiscus wailesii, Craterostigma, Cytisus cantabricus, Desmodesmus, Arundo, Diatom (Skeletonema), Ditylum brightwellii, Eucalyptus globulus, Fraxinus chinensis subsp. Rhynchophylla, Gongolaria barbata, Halomicronema hongdechloris, Lasallia hispanica, Lindernia, Manihot esculenta, Marchantia polymorpha, Megathyrsus maximus, Microcystis aeruginosa

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Blue-native PAGE (BN PAGE), Electron Microscopy (EM), Immunofluorescence (IF)

Détail du produit

Épitope: C-Term

Fonction: Anti-PsbA | D1 protein of PSII, C-terminal (rabbit antibody) (thylakoid membrane marker)

Réactivité croisée (Details): Alniaria alnifolia, Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp., Begonia sp. , Cannabis sativa L., Cladocopium goreaui, Chlamydomonas reinhardtii, Chlorella ohadii, Chromera velia, Chlorella vulgaris, Colobanthus quitensis (Kunth) Bartl, Coscinodiscus wailesii, Craterostigma sp., Cyanidioschyzon merolae, Cytisus cantabricus (Wilk.) Rchb. F, Desmodesmus sp., Dianthus caryophyllus, Ditylum brightwellii, Eucalyptus globulus, Fraxinus rhynchophylla, Glycine max, Gongolaria barbata,Halomicronema hongdechloris, Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Lindernia sp., Manihot esculenta, Marchantia polymorpha (liverwort), Medicago truncatula, Miscanthus x giganteus, Microcystis aeruginosa, Mirkania micrantha, Nicotiana benthamiana, Nicotiana tabcum, Panicum miliaceum, Panax ginseng, Panicum maximum, Paulinella chromatophora (amoeba), Pheodactylum tricornutum CCAP 1055/1, Physcomitrium patens, Picea glauca, Pinus strobus, Pisum sativum, Prochlorococcus sp. (surface and deep water ecotype), Salicornia bigelovii , Skeletonema costatum (diatom), Solanum lycopersicum, Spartina alterniflora, Spinacia oleracea, Spirodela polyrhiza, Symbiodinium sp, Synechococcus sp. PCC 7942, Synechococcus elongatus UTEX 2973, Synechocystis sp. 6803, Syntrichia muralis, Thalassiosira weissflogii, Tetradesmus obliquus, Triticum aestivum, Triticale, Zea may, Quercus ilex
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Algae (brown,red),Brassica napus,Carica papaya,Conifers,Cyanobacteria,Cannabis sativa,Citrus sinensis,Dicots,Eragrostis tef,Galdieria sulphuraria,Lactuca sativa,Lycopersicum esculentum,Medicago sativa,Nannochloropsis sp.,Oryza sativa,Ostreococcus sp.,Phalaenopsis aphrodite,Pisum sativum,Porphyridium purpureum,Sesamum indicum,Thalassiosira pseudonana,Zosteria marina,Vitis vinifera cellular [compartment marker] of thylakoid membrane

Purification: Serum

Immunogène: KLH-conjugated synthetic peptide derived from available plant

Information d'application

Indications d'application: 1: 500 (IF), 1: 200 (IG), 1 : 10 000 (WB)

Commentaires:

The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the hen anti-PsbA antibodies AS01 016.This antibody will detect the phosphorylated form of D1as an alternate band to the main band on a high resolution gel.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Référence

Pandey, Pandey-Rai: "Modulations of physiological responses and possible involvement of defense-related secondary metabolites in acclimation of Artemisia annua L. against short-term UV-B radiation." dans: Planta, Vol. 240, Issue 3, pp. 611-27, (2014) (PubMed).

Lagunas, Román, Andreu, Picorel, Alfonso: "A temporal regulatory mechanism controls the different contribution of endoplasmic reticulum and plastidial ω-3 desaturases to trienoic fatty acid content during leaf development in soybean (Glycine max cv Volania)." dans: Phytochemistry, Vol. 95, pp. 158-67, (2013) (PubMed).

Nowack, Grossman: "Trafficking of protein into the recently established photosynthetic organelles of Paulinella chromatophora." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 14, pp. 5340-5, (2012) (PubMed).

Ueda, Kuniyoshi, Yamamoto, Sugimoto, Ishizaki, Kohchi, Nishimura, Shikanai: "Composition and physiological function of the chloroplast NADH dehydrogenase-like complex in Marchantia polymorpha." dans: The Plant journal : for cell and molecular biology, Vol. 72, Issue 4, pp. 683-93, (2012) (PubMed).

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." dans: Journal of plant physiology, (2011) (PubMed).

Balsemão-Pires, Jaillais, Olson, Andrade, Umen, Chory, Sachetto-Martins: "The Arabidopsis translocator protein (AtTSPO) is regulated at multiple levels in response to salt stress and perturbations in tetrapyrrole metabolism." dans: BMC plant biology, Vol. 11, pp. 108, (2011) (PubMed).

Krieger-Liszkay, Ka: "Superoxide anion radicals generated by methylviologen in photosystem I damage photosystem II." dans: Physiologia plantarum, (2010) (PubMed).

Détail du antigène

Antigène: D1 Protein of PSII, (PsbA)

Sujet:

Background: The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1

Additional information: Due to biology of PsbA (D1) protein a number of degradation products can apprear in a sample and may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24 kDa and 16 kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands.Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.This product can be sold containing ProClin if requested.

Poids moléculaire: 38 | 28-30 kDa

ID gène: 844802

UniProt: P83755, P0C434, Q14FH6, Q6YXN7, P07753, P14660