ABCB1 anticorps

N° du produit ABIN2613685

Cet anticorps anti-ABCB1 Monoclonal Souris (Clone C219) (ABIN2613685) détecte spécifiquement ABCB1 dans WB, IHC, IHC (p), FACS, IP, IHC (fro) et ICC. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat et Hamster.

Aperçu rapide pour ABCB1 anticorps (ABIN2613685)

Antigène: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Reactivité: Humain, Souris, Rat, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ABCB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Clone: C219

Détail du produit anti-ABCB1 anticorps

Specificité: Recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, P-glycoprotein and MDR3 P-glycoprotein, not species specific. Species Crossreactivity: human, mouse, rat and hamster. Crossreactivity Proteins: a ~200kD protein which migrates in the same position as myosin and with C-erbB2 protein (p185c-erbB2).

Purification: Protein A purified

Immunogène: SDS-solubilized plasma membranes of an MDR (multidrug resistant) Chinese hamster ovary (CHO) cell line and a human cell line.

Type of Immunogen: Cell fraction

Isotype: IgG1

Information d'application

Indications d'application: Approved: Flo, ICC, IHC, IHC-Fr, IHC-P, IP, WB (1:25 - 1:50)

Usage: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Flow Cytometry: Cell permeabilization required. Western Blot: 1:25-1:50. Immunohistochemistry (Paraffin and Frozen): For paraffin sections, dilute 1:12.5 for biotin based detection. The antibody should be incubated for 60 minutes at RT. Immunocytochemistry: Cytospin preparations. Positive control: Cell lines: Appropriate drug-sensitive parental cell lines and their multidrug resistant derivatives prepared by the same methods as the test specimens can be used as control materials.

Commentaires:

Target Species of Antibody: Hamster

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 % BSA, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur ABCB1

Antigène: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Autre désignation: ABCB1 / MDR1 / P Glycoprotein

Sujet: Name/Gene ID: ABCB1
Subfamily: ATP-binding cassette - ABCB/MDR
Family: Transporter

Synonyms: ABCB1, ABC20, Abcb1b, CLCS, Colchicin sensitivity, CD243, Doxorubicin resistance, IBD13, gp170, MDR1, Multidrug resistance protein 1, P glycoprotein, P-glycoprotein 1, P-GP, CD243 antigen, PGY1

ID gène: 5243

UniProt: P08183