Cet anticorps anti-GAPDH Monoclonal Souris (Clone B903M) (ABIN263739) détecte spécifiquement GAPDH dans WB, IF et EIA.
L’anticorps est réactif avec des échantillons de Humain, Rat, Lapin, Porc, Boeuf (Vache) et Chévre.
GAPDH
Reactivité: Humain
WB, ELISA, IHC, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA. Western Blot. Immunocytochemistry. Recommended pairs for Sandwich Immunoassay: Capture: ABIN263741Detection: ABIN263739 Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
4.5 mg/mL (Sigma protein assay kit)
Buffer
PBS, pH 7.4, 0.09 % Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C. Shelf life: One year from despatch.
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36 kDa subunits.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase