GAPDH anticorps

N° du produit ABIN263740

Cet anticorps Souris Monoclonal détecte spécifiquement GAPDH dans WB et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Lapin, Porc, Chien, Boeuf (Vache), Poisson, Cat et Chévre.

Aperçu rapide pour GAPDH anticorps (ABIN263740)

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité: Humain, Souris, Rat, Lapin, Porc, Chien, Boeuf (Vache), Poisson, Cat, Chévre

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GAPDH est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 4G5

Détail du produit anti-GAPDH anticorps

Specificité: This antibody recognizes GAPDH monomer (36 kDa) and the dimer form. Does not react with the tetrameric form.

Attributs du produit: Synonyms: GAPD, CDABP0047, Loading control, Glyceraldehyde-3-Phosphate Dehydrogenase

Purification: Protein A Chromatography.

Immunogène: Rabbit muscle GAPDH. Hybridization of Sp2/0 myeloma cells with spleen cells from BALB/cmice.

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 4.1 mg/mL (Sigma protein assay kit)

Buffer: PBS, pH 7.4 containing 0,09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur GAPDH

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: GAPDH

Sujet: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase

ID gène: 2597

UniProt: P04406