LEF1 anticorps (HMG Binding Domain)

N° du produit ABIN264381

L’anticorps Souris Monoclonal anti-LEF1 a été validé pour WB et IF. Il convient pour détecter LEF1 dans des échantillons de Humain et Souris.

Aperçu rapide pour LEF1 anticorps (HMG Binding Domain) (ABIN264381)

Antigène: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LEF1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: REMB6

Détail du produit anti-LEF1 anticorps

Épitope: HMG Binding Domain

Specificité: REMB6 recognizes the High Mobility Group DNA domain of LEF-1.

Attributs du produit: Synonyms: Lymphoid enhancer-binding factor 1, LEF-1, T cell-specific transcription factor 1-alpha,TCF1-alpha

Purification: Purified

Immunogène: Full-length recombinant LEF-1 protein. Genename: LEF1

Isotype: IgG1

Information d'application

Indications d'application: Western Blot: 1-5 μg/mLImmunofluorescence on Paraformaldehyde-Fixed Cells: 1 μg/mL (1/1000). Positive Control: Jurkat cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS, 0.08 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur LEF1

Antigène: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Autre désignation: LEF1

Sujet: Lymphoid Enhancer Factor-1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms.Synonyms: LEF-1, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha

ID gène: 51176

UniProt: Q9UJU2

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding