His Tag anticorps

N° du produit ABIN264410

L’anticorps anti-His Tag Monoclonal Souris est utilisé pour la détection de His Tag. Il a été validé pour WB.

Aperçu rapide pour His Tag anticorps (ABIN264410)

Antigène: His Tag

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est non-conjugé

Application: Western Blotting (WB)

Clone: IPA2C5-1

Détail du produit anti-His Tag anticorps

Séquence: HHHHHH

Attributs du produit: Synonyms: 6xHis-Tag, His Tag, HIS6 Tag, HHHHHH Tag

Purification: Purified

Immunogène: Hybridoma produced by the fusion of splenocytes from mice immunized withHis-His-His-His-His-His synthetic peptide and mouse myeloma cells.

Isotype: IgG2a

Information d'application

Indications d'application: Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur His Tag

Antigène: His Tag

Autre désignation: 6xHistidine Epitope Tag

Classe de substances: Tag

Sujet: 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.