ATIC anticorps

N° du produit ABIN264511

Cet anticorps Souris Monoclonal détecte spécifiquement ATIC dans WB et IHC (fro). Il présente une réactivité envers Humain, Souris, Rat, Drosophila melanogaster et Grenouille.

Aperçu rapide pour ATIC anticorps (ABIN264511)

Antigène: ATIC (5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC))

Reactivité: Humain, Souris, Rat, Drosophila melanogaster, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ATIC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: F38 P7 H9

Détail du produit anti-ATIC anticorps

Specificité: This antibody detects Phosphoribosylaminoimidazolecarboxamide Formyltransferase (ATIC).

Attributs du produit: Synonyms: IMP cyclohydrolase, Inosinicase, IMP synthetase, Bifunctional purine biosynthesis proteinPURH

Purification: Affinity chromatography.

Immunogène: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with asynthetic peptide derived from the human ATIC protein and mouse myeloma Ag8563 cells. Sequence common in Frog, Fruit fly, Rat and Mouse.

Isotype: IgG1

Information d'application

Indications d'application: Western Blot: 1-2 μg/mL. Immunohistochemistry on Paraffin Sections (15 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1 x TBSwithTween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur ATIC

Antigène: ATIC (5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC))

Autre désignation: ATIC / PURH

Sujet: The bifunctional purine biosynthesis protein PURH contains phosphoribosylaminoimidazole carboxamide formyltransferase, also designated AICAR transformylase, IMP cyclohydrolase or Inosinicase. AICAR plays an important role in purine biosynthesis, specifically in the production of nucleotides and IMP. Defects in ATIC, the gene encoding for this protein, can cause AICArebosuria, also designated AICA-ribosiduria, an inborn error in purine biosynthesis that is neurologically cataclysmic. Individuals with AICA-rebosuria accumulate AICA-ribotide, also designated ZMP, and its derivatives in erythrocytes and fibroblasts and also excrete very large amounts of AICA-riboside in the urine. Mental retardation, epilepsy, dysmorphic features and congenital blindness are all symptoms of this disease.Synonyms: Bifunctional purine biosynthesis protein PURH, IMP cyclohydrolase, IMP synthetase, Inosinicase

ID gène: 471

UniProt: P31939