Nucleolin anticorps

N° du produit ABIN2668241

Cet anticorps anti-Nucleolin Monoclonal Souris (Clone 3G4B20) (ABIN2668241) détecte spécifiquement Nucleolin dans WB, IF et ICC. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour Nucleolin anticorps (ABIN2668241)

Antigène: Nucleolin (NCL)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nucleolin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 3G4B20

Détail du produit anti-Nucleolin anticorps

Fonction: Nucleolin antibody (mAb) (Clone 3G4B20)

Purification: Culture Supernatant

Immunogène: This Nucleolin antibody was raised against a salt-extracted nuclear matrix preparation from MDCK cells.

Isotype: IgG1

Information d'application

Indications d'application: ICC/IF: 1:500 dilution WB*: 1:500 - 1:2,000 dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91231. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Concentrated culture supernatant containing 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur Nucleolin

Antigène: Nucleolin (NCL)

Autre désignation: Nucleolin

Sujet: Nucleolin (NCL, C23) is an abundant and ubiquitously expressed protein that is a major component of the nucleolus. Nucleolin seems to be over-expressed in highly proliferative cells and is involved in many aspects of gene expression: chromatin remodeling, DNA recombination and replication, RNA transcription by RNA polymerase I and II (RNA pol II), rRNA processing, mRNA stabilization, cytokinesis and apoptosis. Its role as a histone chaperone has recently been identified. It is involved in the synthesis and maturation of ribosomes, and is located mainly in dense fibrillar regions of the nucleolus.

Poids moléculaire: 100 kDa

ID gène: 4691

NCBI Accession: XP_850477

Pathways: Ribonucleoprotein Complex Subunit Organization