HDAC4 anticorps (AA 194-209)

N° du produit ABIN2668313

Cité dans 9 publications. Cet anticorps anti-HDAC4 Polyclonal Lapin (ABIN2668313) détecte spécifiquement HDAC4 dans WB, ChIP et ChIP-seq. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour HDAC4 anticorps (AA 194-209) (ABIN2668313)

Antigène: HDAC4 (Histone Deacetylase 4 (HDAC4))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HDAC4 est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Détail du produit anti-HDAC4 anticorps

Épitope: AA 194-209

Fonction: HDAC4 antibody (pAb)

Purification: Affinity Purified

Immunogène: This HDAC4 antibody was raised against a synthetic peptide corresponding to amino acid residues 194-209 of human HDAC4.

Isotype: IgG

Information d'application

Indications d'application: ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 - 10 μg each WB: 1 - 2 μg/mL dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 μg/μL

Buffer: PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: 4 °C,-20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-HDAC4 (ABIN2668313)

Liu, Gu, Feng, Yang, Zhu, Lu, Qi: "Both HDAC5 and HDAC6 are required for the proliferation and metastasis of melanoma cells." dans: Journal of translational medicine, Vol. 14, pp. 7, (2016) (PubMed).

Isaacs, Antony, Dalrymple, Brennen, Gerber, Hammers, Wissing, Kachhap, Luo, Xing, Björk, Olsson, Björk, Leanderson: "Tasquinimod Is an Allosteric Modulator of HDAC4 survival signaling within the compromised cancer microenvironment." dans: Cancer research, Vol. 73, Issue 4, pp. 1386-99, (2013) (PubMed).

Keedy, Archin, Gates, Espeseth, Hazuda, Margolis: "A limited group of class I histone deacetylases acts to repress human immunodeficiency virus type 1 expression." dans: Journal of virology, Vol. 83, Issue 10, pp. 4749-56, (2009) (PubMed).

Mottet, Bellahcène, Pirotte, Waltregny, Deroanne, Lamour, Lidereau, Castronovo: "Histone deacetylase 7 silencing alters endothelial cell migration, a key step in angiogenesis." dans: Circulation research, Vol. 101, Issue 12, pp. 1237-46, (2007) (PubMed).

Sayeed, Konduri, Liu, Bansal, Li, Das: "Estrogen receptor alpha inhibits p53-mediated transcriptional repression: implications for the regulation of apoptosis." dans: Cancer research, Vol. 67, Issue 16, pp. 7746-55, (2007) (PubMed).

Testoni, Mantovani: "Mechanisms of transcriptional repression of cell-cycle G2/M promoters by p63." dans: Nucleic acids research, Vol. 34, Issue 3, pp. 928-38, (2006) (PubMed).

Basile, Mantovani, Imbriano: "DNA damage promotes histone deacetylase 4 nuclear localization and repression of G2/M promoters, via p53 C-terminal lysines." dans: The Journal of biological chemistry, Vol. 281, Issue 4, pp. 2347-57, (2006) (PubMed).

Imbriano, Gurtner, Cocchiarella, Di Agostino, Basile, Gostissa, Dobbelstein, Del Sal, Piaggio, Mantovani: "Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters." dans: Molecular and cellular biology, Vol. 25, Issue 9, pp. 3737-51, (2005) (PubMed).

Caretti, Salsi, Vecchi, Imbriano, Mantovani: "Dynamic recruitment of NF-Y and histone acetyltransferases on cell-cycle promoters." dans: The Journal of biological chemistry, Vol. 278, Issue 33, pp. 30435-40, (2003) (PubMed).

Détails sur HDAC4

Antigène: HDAC4 (Histone Deacetylase 4 (HDAC4))

Autre désignation: HDAC4

Sujet: HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.

Poids moléculaire: 140 kDa

ID gène: 9759

NCBI Accession: NP_006028

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process