Histone 3 anticorps (H3K4me2)

N° du produit ABIN2668392

Cet anticorps Souris Monoclonal détecte spécifiquement Histone 3 dans WB, IF, ChIP, ICC, DB et ChIP-seq. Il présente une réactivité avec des échantillons de Humain et Souris. Il a été cité dans 2+ publications.

Aperçu rapide pour Histone 3 anticorps (H3K4me2) (ABIN2668392)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Clone: MABI 0303

Détail du produit anti-Histone 3 anticorps

Épitope: H3K4me2

Fonction: Histone H3K4me2 antibody (mAb) (Clone MABI 0303)

Purification: Protein G Chromatography

Immunogène: This Histone H3 dimethyl Lys4 antibody was raised against a synthetic peptide containing dimethyl Lys4 of human histone H3.

Isotype: IgG1

Information d'application

Indications d'application: ChIP: 5 - 10 μg per ChIP ChIP-Seq: 5 - 10 μg each ICC/IF: 1 - 2 µL/mL dilution WB: 2 - 5 μg/mL dilution DB: 1 μg/mL dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here. For Histone H3K4me2, we also offer AbFlex Histone H3K4me2 Recombinant Antibody (rAb). For details, see Catalog No. 91321.

Restrictions: For Research Use only

Stockage

Concentration: 0.46 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol, 0.3 M NaCl, and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone 3 (ABIN2668392)

Valdez, Li, Murray, Ji, Liu, Popat, Champlin, Andersson: "Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators." dans: Experimental hematology, Vol. 43, Issue 6, pp. 448-61.e2, (2015) (PubMed).

Kimura, Hayashi-Takanaka, Goto, Takizawa, Nozaki: "The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies." dans: Cell structure and function, Vol. 33, Issue 1, pp. 61-73, (2008) (PubMed).

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

Poids moléculaire: 17 kDa

ID gène: 3020

NCBI Accession: NP_003522