Histone 3 anticorps (H3R8me2a)

N° du produit ABIN2668406

Cité dans 2 publications. Cet anticorps anti-Histone 3 Polyclonal Lapin (ABIN2668406) détecte spécifiquement Histone 3 dans WB, ChIP, DB et ChIP-seq. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour Histone 3 anticorps (H3R8me2a) (ABIN2668406)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Détail du produit anti-Histone 3 anticorps

Épitope: H3R8me2a

Fonction: Histone H3R8me2a (asymmetric) antibody (pAb)

Purification: Affinity Purified

Immunogène: This Histone H3 dimethyl Arg8 asymmetric antibody was raised against a peptide containing dimethyl-Arg8 (asymmetric) of human histone H3.

Isotype: IgG

Information d'application

Indications d'application: ChIP: 10 µL per ChIP ChIP-Seq: 10 µL each WB: 1:500 dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone 3 (ABIN2668406)

Papazyan, Voronina, Chapman, Luperchio, Gilbert, Meier, Mackintosh, Shabanowitz, Tackett, Reddy, Coyne, Hunt, Liu, Taverna: "Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis." dans: eLife, Vol. 3, pp. e02996, (2014) (PubMed).

Blythe, Cha, Tadjuidje, Heasman, Klein: "beta-Catenin primes organizer gene expression by recruiting a histone H3 arginine 8 methyltransferase, Prmt2." dans: Developmental cell, Vol. 19, Issue 2, pp. 220-31, (2010) (PubMed).

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation.

Poids moléculaire: 17 kDa

ID gène: 3020

NCBI Accession: NP_003522