Histone 3 anticorps (H3K18ac)

N° du produit ABIN2668428

Cet anticorps Lapin Polyclonal détecte spécifiquement Histone 3 dans WB, IF, ChIP, ICC, DB et ChIP-seq. Il présente une réactivité avec des échantillons de Humain. Il a été cité dans 3+ publications.

Aperçu rapide pour Histone 3 anticorps (H3K18ac) (ABIN2668428)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Détail du produit anti-Histone 3 anticorps

Épitope: H3K18ac

Fonction: Histone H3K18ac antibody (pAb)

Purification: Protein A Chromatography

Immunogène: This Histone H3 acetyl Lys18 antibody was raised against a peptide including acetyl-lysine 18 of histone H3.

Isotype: IgG

Information d'application

Indications d'application: ChIP: 10 μg per ChIP ChIP-Seq: 4 μg each ICC/IF: 1 μg/mL dilution WB: 0.5 - 2 μg/mL dilution DB: 0.1 - 0.5 μg/mL dilution

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone 3 (ABIN2668428)

Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." dans: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

Yasuma, Yasunaga, Takemoto, Sugata, Mitobe, Takenouchi, Nakagawa, Suzuki, Matsuoka: "HTLV-1 bZIP Factor Impairs Anti-viral Immunity by Inducing Co-inhibitory Molecule, T Cell Immunoglobulin and ITIM Domain (TIGIT)." dans: PLoS pathogens, Vol. 12, Issue 1, pp. e1005372, (2016) (PubMed).

Cohen, Friedmann, Hwang, Marmorstein, Lee: "The microtubule-associated tau protein has intrinsic acetyltransferase activity." dans: Nature structural & molecular biology, Vol. 20, Issue 6, pp. 756-62, (2013) (PubMed).

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.

Poids moléculaire: 17 kDa

ID gène: 3020

NCBI Accession: NP_003522