Histone 3 anticorps (H3K27me2)

N° du produit ABIN7540290

Il existe 6+ publications pour ce produit. L’anticorps anti-Histone 3 Polyclonal Lapin est utilisé pour la détection de Histone 3 dans des échantillons de Humain. Il a été validé pour WB, IF, ChIP, ICC, DB et ChIP-seq.

Aperçu rapide pour Histone 3 anticorps (H3K27me2) (ABIN7540290)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Détail du produit anti-Histone 3 anticorps

Épitope: H3K27me2

Fonction: Histone H3K27me2 antibody (pAb)

Purification: Unpurified

Immunogène: This Histone H3 dimethyl Lys27 antibody was raised against a peptide including dimethyl-lysine 27 of histone H3.

Information d'application

Indications d'application: ChIP-Seq: 4-6 µL per ChIP ChIP: 5 - 10 µL per ChIP ICC/IF: 1:200 - 1:500 dilution WB: 1:500 - 1:1,000 dilution

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone 3 (ABIN7540290)

Xiao, Shi, Fan, Zhang, Wu, Lan, Minze, Fu, Ghobrial, Liu, Li: "GITR subverts Foxp3(+) Tregs to boost Th9 immunity through regulation of histone acetylation." dans: Nature communications, Vol. 6, pp. 8266, (2015) (PubMed).

Herz, Morgan, Gao, Jackson, Rickels, Swanson, Florens, Washburn, Eissenberg, Shilatifard: "Histone H3 lysine-to-methionine mutants as a paradigm to study chromatin signaling." dans: Science (New York, N.Y.), Vol. 345, Issue 6200, pp. 1065-70, (2014) (PubMed).

Cie?lik, Hoang, Baranova, Chodaparambil, Kumar, Allison, Xu, Wamsley, Gray, Jones, Mayo, Bekiranov: "Epigenetic coordination of signaling pathways during the epithelial-mesenchymal transition." dans: Epigenetics & chromatin, Vol. 6, Issue 1, pp. 28, (2013) (PubMed).

Boulay, Dubuissez, Van Rechem, Forget, Helin, Ayrault, Leprince: "Hypermethylated in cancer 1 (HIC1) recruits polycomb repressive complex 2 (PRC2) to a subset of its target genes through interaction with human polycomb-like (hPCL) proteins." dans: The Journal of biological chemistry, Vol. 287, Issue 13, pp. 10509-24, (2012) (PubMed).

Lafos, Kroll, Hohenstatt, Thorpe, Clarenz, Schubert: "Dynamic regulation of H3K27 trimethylation during Arabidopsis differentiation." dans: PLoS genetics, Vol. 7, Issue 4, pp. e1002040, (2011) (PubMed).

Qi, Sarkissian, Hu, Wang, Bhattacharjee, Gordon, Gonzales, Lan, Ongusaha, Huarte, Yaghi, Lim, Garcia, Brizuela, Zhao, Roberts, Shi: "Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial development." dans: Nature, Vol. 466, Issue 7305, pp. 503-7, (2010) (PubMed).

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H3K27 can be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.

Poids moléculaire: 17 kDa

ID gène: 3020

NCBI Accession: NP_003522