Histone 3 anticorps (H3K4me3)

N° du produit ABIN2668474

Il existe 8+ publications pour ce produit. L’anticorps anti-Histone 3 Polyclonal Lapin est utilisé pour la détection de Histone 3 dans des échantillons de Humain et Saccharomyces cerevisiae. Il a été validé pour WB, IF, ChIP, ICC, DB, ChIP-seq et CUT&RUN.

Aperçu rapide pour Histone 3 anticorps (H3K4me3) (ABIN2668474)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain, Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Détail du produit anti-Histone 3 anticorps

Épitope: H3K4me3

Fonction: Histone H3K4me3 antibody (pAb)

Purification: Protein A Chromatography

Immunogène: This Histone H3 trimethyl Lys4 antibody was raised against a peptide including trimethyl-lysine 4 of histone H3.

Isotype: IgG

Information d'application

Indications d'application: ChIP: 3 μg per ChIP ChIP-Seq: 4 μg each ICC/IF: 1 - 2 μg/mL dilution WB*: 2 - 4 μg/mL dilution CUT&Tag: 1 µL per 50 µL reaction* CUT&RUN: 1 µL per 50 µL reaction *This antibody has been validated for CUT&Tag using Active Motif's CUT&Tag-IT Assay Kit, Catalog No. 53160. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone 3 (ABIN2668474)

Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." dans: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

Akiyama, Koda, Byeon, Shimada, Nishikawaji, Sakamoto, Chen, Kojima, Kawano, Eishi, Deng, Kim, Zhu, Yuasa, Tanaka: "Reduced expression of SET7/9, a histone mono-methyltransferase, is associated with gastric cancer progression." dans: Oncotarget, Vol. 7, Issue 4, pp. 3966-83, (2016) (PubMed).

Singh, Johnstone, Martin, Tempera, Kaplan, Denny: "Alterations in nuclear structure promote lupus autoimmunity in a mouse model." dans: Disease models & mechanisms, Vol. 9, Issue 8, pp. 885-97, (2016) (PubMed).

Heubach, Monsior, Deenen, Niegisch, Szarvas, Niedworok, Schulz, Hoffmann: "The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells." dans: Molecular cancer, Vol. 14, pp. 108, (2015) (PubMed).

Cabrera, Olcese, Horabin: "A balancing act: heterochromatin protein 1a and the Polycomb group coordinate their levels to silence chromatin in Drosophila." dans: Epigenetics & chromatin, Vol. 8, pp. 17, (2015) (PubMed).

Sakashita, Kawabata, Jincho, Tajima, Kumamoto, Kobayashi, Matsui, Kono: "Sex Specification and Heterogeneity of Primordial Germ Cells in Mice." dans: PLoS ONE, Vol. 10, Issue 12, pp. e0144836, (2015) (PubMed).

Walter, Matter, Fahrenkrog: "Loss of histone H3 methylation at lysine 4 triggers apoptosis in Saccharomyces cerevisiae." dans: PLoS genetics, Vol. 10, Issue 1, pp. e1004095, (2014) (PubMed).

DesRochers, Shamis, Alt-Holland, Kudo, Takata, Wang, Jackson-Grusby, Garlick: "The 3D tissue microenvironment modulates DNA methylation and E-cadherin expression in squamous cell carcinoma." dans: Epigenetics, Vol. 7, Issue 1, pp. 34-46, (2012) (PubMed).

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

Poids moléculaire: 17 kDa

ID gène: 3020

NCBI Accession: NP_003522