TET3 anticorps (AA 1159-1329)

N° du produit ABIN2668513

Il existe 2+ publications pour ce produit. L’anticorps anti-TET3 Polyclonal Lapin est utilisé pour la détection de TET3 dans des échantillons de Souris. Il a été validé pour WB et ChIP.

Aperçu rapide pour TET3 anticorps (AA 1159-1329) (ABIN2668513)

Antigène: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TET3 est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Détail du produit anti-TET3 anticorps

Épitope: AA 1159-1329

Fonction: Tet3 antibody (pAb)

Purification: Unpurified

Immunogène: This antibody was raised against a recombinant protein corresponding to amino acids 1159-1329 of mouse Tet3.

Information d'application

Indications d'application: WB: 1:1,000 - 1:5,000 dilution

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-TET3 (ABIN2668513)

Thienpont, Steinbacher, Zhao, DAnna, Kuchnio, Ploumakis, Ghesquière, Van Dyck, Boeckx, Schoonjans, Hermans, Amant, Kristensen, Koh, Mazzone, Coleman, Carell, Carmeliet, Lambrechts: "Tumour hypoxia causes DNA hypermethylation by reducing TET activity." dans: Nature, Vol. 537, Issue 7618, pp. 63-68, (2016) (PubMed).

Otani, Kimura, Sharif, Endo, Mishima, Kawakami, Koseki, Shirakawa, Suetake, Tajima: "Cell cycle-dependent turnover of 5-hydroxymethyl cytosine in mouse embryonic stem cells." dans: PLoS ONE, Vol. 8, Issue 12, pp. e82961, (2013) (PubMed).

Détails sur TET3

Antigène: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Autre désignation: Tet3

Sujet: Tet3 (tet methylcytosine dioxygenase 3) catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Conversion into 5hmC initiates a process leading to cytosine demethylation through deamination into 5-hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC.

Poids moléculaire: 200 kDa

ID gène: 194388

NCBI Accession: NP_898961

Pathways: L'effet Warburg