TWIST1 anticorps

N° du produit ABIN2668516

Il existe 5+ publications pour ce produit. L’anticorps anti-TWIST1 Monoclonal Souris est utilisé pour la détection de TWIST1 dans des échantillons de Humain et Souris. Il a été validé pour WB, IHC, ICC et IF.

Aperçu rapide pour TWIST1 anticorps (ABIN2668516)

Antigène: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TWIST1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-TWIST1 anticorps

Fonction: TWIST antibody (mAb)

Purification: Protein G Chromatography

Immunogène: This TWIST antibody was raised against a recombinant protein corresponding to full-length mouse TWIST.

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-TWIST1 (ABIN2668516)

Ezponda, Popovic, Shah, Martinez-Garcia, Zheng, Min, Will, Neri, Kelleher, Yu, Licht: "The histone methyltransferase MMSET/WHSC1 activates TWIST1 to promote an epithelial-mesenchymal transition and invasive properties of prostate cancer." dans: Oncogene, Vol. 32, Issue 23, pp. 2882-90, (2013) (PubMed).

Casas, Kim, Bendesky, Ohno-Machado, Wolfe, Yang: "Snail2 is an essential mediator of Twist1-induced epithelial mesenchymal transition and metastasis." dans: Cancer research, Vol. 71, Issue 1, pp. 245-54, (2011) (PubMed).

Eckert, Lwin, Chang, Kim, Danis, Ohno-Machado, Yang: "Twist1-induced invadopodia formation promotes tumor metastasis." dans: Cancer cell, Vol. 19, Issue 3, pp. 372-86, (2011) (PubMed).

Kida, Asahina, Teraoka, Gitelman, Sato: "Twist relates to tubular epithelial-mesenchymal transition and interstitial fibrogenesis in the obstructed kidney." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 55, Issue 7, pp. 661-73, (2007) (PubMed).

Gitelman: "Twist protein in mouse embryogenesis." dans: Developmental biology, Vol. 189, Issue 2, pp. 205-14, (1997) (PubMed).

Détails sur TWIST1

Antigène: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Autre désignation: TWIST

Sujet: TWIST is a regulator of transcription that serves as a master regulator of embryonic morphogenesis. Through its bHLH motif it interacts with many different transcription factors to affect multiple pathways of gene expression. TWIST plays a pivotal role in mesodermal, myoblast, and osteoblast differentiation. TWIST is involved in the epithelial-mesenchymal transition (EMT), a period during embryonic development characterized by loss of cell adhesion and increased cell mobility, essential events for mesoderm and neural tube formation. The EMT shares many features of metastatic cancer, and elevated levels of TWIST are observed in many types of cancer. Mutations in the gene encoding TWIST have been associated with Saethre-Chotzen syndrome, an inherited developmental disorder characterized by skull deformations, limb abnormalities, and facial dysmorphisim.

Poids moléculaire: 21 kDa

ID gène: 7291

NCBI Accession: NP_000465

Pathways: Signalisation p53, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity