Rpb1 CTD anticorps (Thr4)

N° du produit ABIN2668543

Il existe 4+ publications pour ce produit. L’anticorps anti-Rpb1 CTD Monoclonal Rat est utilisé pour la détection de Rpb1 CTD dans des échantillons de Humain. Il a été validé pour WB, IF, ICC et ChIP.

Aperçu rapide pour Rpb1 CTD anticorps (Thr4) (ABIN2668543)

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Rpb1 CTD est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Clone: 6D7

Détail du produit anti-Rpb1 CTD anticorps

Épitope: Thr4

Purification: Protein G Chromatography

Immunogène: This antibody was raised against a synthetic peptide corresponding to RNA Pol II CTD phosphorylated at threonine 4.

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation:

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Stock: -20 °C

Stockage commentaire: Antibodies in solution can be stored at -20 °C for 2 years.

Date de péremption: 6 months

Références pour anticorps anti-Rpb1 CTD (ABIN2668543)

Harlen, Trotta, Smith, Mosaheb, Fuchs, Churchman: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." dans: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).

Laitem, Zaborowska, Isa, Kufs, Dienstbier, Murphy: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." dans: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).

Hintermair, Heidemann, Koch, Descostes, Gut, Gut, Fenouil, Ferrier, Flatley, Kremmer, Chapman, Andrau, Eick: "Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation." dans: The EMBO journal, Vol. 31, Issue 12, pp. 2784-97, (2012) (PubMed).

Mayer, Heidemann, Lidschreiber, Schreieck, Sun, Hintermair, Kremmer, Eick, Cramer: "CTD tyrosine phosphorylation impairs termination factor recruitment to RNA polymerase II." dans: Science (New York, N.Y.), Vol. 336, Issue 6089, pp. 1723-5, (2012) (PubMed).

Détails sur Rpb1 CTD

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Autre désignation: RNA Pol II CTD

Poids moléculaire: 240 kDa

ID gène: 5430