Recombinant Rpb1 CTD anticorps (pTyr1) (Sortase,His tag,AVI tag)

N° du produit ABIN6731014

L’anticorps anti-Rpb1 CTD Monoclonal Souris est utilisé pour la détection de Rpb1 CTD dans des échantillons de Humain. Il a été validé pour WB, IF, ChIP-seq et ELISA.

Aperçu rapide pour Recombinant Rpb1 CTD anticorps (pTyr1) (Sortase,His tag,AVI tag) (ABIN6731014)

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Rpb1 CTD est conjugé à/à la Sortase,His tag,AVI tag

Application: Western Blotting (WB), Immunofluorescence (IF), ChIP DNA-Sequencing (ChIP-seq), ELISA

Détail du produit anti-Rpb1 CTD anticorps (Sortase,His tag,AVI tag)

Épitope: pTyr1

Fonction: AbFlex RNA Pol II CTD phospho Tyr1 antibody (rAb)

Marque: AbFlex®

Attributs du produit: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

Purification: Protein A Chromatography

Immunogène: This antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.

Isotype: IgG2a

Information d'application

Indications d'application: ChIP-Seq: 4-10 μg per ChIP IF: 0.5 - 2 μg/mL Bead-based ELISA: 0.5 - 10 μg/mL WB*: 1 - 2 μg/mL For optimal results, we recommend the addition of 0.1 % Tween 20 to all blocking solutions to reduce background. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61383.

Commentaires:

This recombinant antibody has been generated using defined DNA sequences to achieve high specificity and high reproducibility. The unique advantages of this antibody are its flexible labeling and purification options as it contains a sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner. Every antibody also contains a 6xHis Tag, which can be used with nickel-based purification systems, and a Biotinylation Tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur Rpb1 CTD

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Autre désignation: RNA Pol II CTD

Sujet: AbFlex RNA Pol II CTD phospho Tyr1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Poids moléculaire: 210 kDa

NCBI Accession: NP_000928