Rpb1 CTD anticorps (pSer2)

N° du produit ABIN2668545

Cet anticorps Lapin Polyclonal détecte spécifiquement Rpb1 CTD dans WB et IP. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Rpb1 CTD anticorps (pSer2) (ABIN2668545)

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Rpb1 CTD est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-Rpb1 CTD anticorps

Épitope: pSer2

Fonction: RNA pol II CTD phospho Ser2 antibody (pAb)

Purification: Affinity Purified

Immunogène: This RNA pol II CTD phospho Ser2 antibody was raised against a peptide containing the RNA Pol II CTD sequence phosphorylated at serine 2.

Isotype: IgG

Information d'application

Indications d'application: IP: 5 - 10 µL per IP WB: 1:500 - 1:2,000 dilution For RNA pol II CTD phospho Ser2, we also offer AbFlex RNA pol II CTD phospho Ser2 Recombinant Antibody (rAb). For details, see Catalog No. 91115.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur Rpb1 CTD

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Autre désignation: RNA Pol II CTD

Sujet: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation.

Poids moléculaire: 240 kDa

ID gène: 5430

NCBI Accession: NP_000928