BMI1 anticorps

N° du produit ABIN2668619

Cité dans 2 publications. Cet anticorps anti-BMI1 Monoclonal Souris (Clone AF27) (ABIN2668619) détecte spécifiquement BMI1 dans WB, IHC, IF, ChIP-seq et ChIP. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour BMI1 anticorps (ABIN2668619)

Antigène: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BMI1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP)

Clone: AF27

Détail du produit anti-BMI1 anticorps

Fonction: BMI-1 antibody (mAb) (Clone AF27)

Purification: Protein G Chromatography

Immunogène: This BMI-1 antibody was raised against a recombinant protein corresponding to full-length mouse BMI-1.

Isotype: IgG2a

Information d'application

Indications d'application: ChIP-Seq: 4 μg per ChIP WB: 0.5 - 2 μg/mL For optimal results, we recommend the addition of 0.05 % Tween 20 to all blocking solutions to reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-BMI1 (ABIN2668619)

Jin, Lee, Heo, Lim, Kim, Kim, Nam, Hong, Cho, Choi, Kim, Shin, Kim: "Senescence associated MCP-1 secretion is dependent on a decline in BMI1 in human mesenchymal stromal cells." dans: Antioxidants & redox signaling, (2015) (PubMed).

Lafkas, Rodilla, Huyghe, Mourao, Kiaris, Fre: "Notch3 marks clonogenic mammary luminal progenitor cells in vivo." dans: The Journal of cell biology, Vol. 203, Issue 1, pp. 47-56, (2013) (PubMed).

Détails sur BMI1

Antigène: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Autre désignation: BMI-1

Sujet: BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.

Poids moléculaire: 40 kDa

ID gène: 6605

NCBI Accession: NP_031578

Pathways: Cycle Cellulaire, Autophagy