RNF2 anticorps

N° du produit ABIN7938435

Cet anticorps Souris Monoclonal détecte spécifiquement RNF2 dans WB, IF, IP, ChIP, ICC et ChIP-seq. Il présente une réactivité avec des échantillons de Humain, Souris et Singe. Il a été cité dans 4+ publications.

Aperçu rapide pour RNF2 anticorps (ABIN7938435)

Antigène: RNF2 (Ring Finger Protein 2 (RNF2))

Reactivité: Humain, Souris, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RNF2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Clone: 3-3

Détail du produit anti-RNF2 anticorps

Fonction: Ring1B antibody (mAb) (Clone 3-3)

Purification: Protein G Chromatography

Immunogène: This Ring1B antibody was raised against the recombinant full-length mouse protein.

Isotype: IgG2b

Information d'application

Indications d'application: ChIP-Seq: 4 µL each

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-RNF2 (ABIN7938435)

Jacinto, Benner, Hetzer: "The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing." dans: Genes & development, Vol. 29, Issue 12, pp. 1224-38, (2015) (PubMed).

Basu, Wilkinson, Colavita, Fennelly, Atchison: "YY1 DNA binding and interaction with YAF2 is essential for Polycomb recruitment." dans: Nucleic acids research, Vol. 42, Issue 4, pp. 2208-23, (2014) (PubMed).

Lin, Shen, Li, Tang, Gu, Chen, Hu, Rice, Lu, Wu: "Proteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program." dans: The Journal of biological chemistry, Vol. 288, Issue 9, pp. 6238-47, (2013) (PubMed).

Lavial, Bessonnard, Ohnishi, Tsumura, Chandrashekran, Fenwick, Tomaz, Hosokawa, Nakayama, Chambers, Hiiragi, Chazaud, Azuara: "Bmi1 facilitates primitive endoderm formation by stabilizing Gata6 during early mouse development." dans: Genes & development, Vol. 26, Issue 13, pp. 1445-58, (2012) (PubMed).

Détails sur RNF2

Antigène: RNF2 (Ring Finger Protein 2 (RNF2))

Autre désignation: Ring1B

Sujet: Ring1B (also known as ring finger protein 2, RING2 or RNF2) is an essential component of the Polycomb group (PcG) multiprotein PRC1 complex. The PRC1 complex is important for the transcriptional repression of genes involved in development and cell proliferation, such as the Hox genes. Ring1B also acts as an E3 ubiquitin ligase on histone H2A of the PRC1 complex.

Poids moléculaire: 40 kDa

ID gène: 6045

NCBI Accession: NP_009143