A2B5 anticorps

N° du produit ABIN2688840

Détail du produit

Antigène: A2B5

Reactivité: Poulet, Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF), BioImaging (BI), Cytotoxicity Test (CyTox)

Marque: BD Pharmingen™

Attributs du produit: The 105/A2B5 monoclonal antibody binds to gangliosides in neural tissue in the retina, brain, spinal cord, and dorsal root ganglia. The antigenic gangliosides are found on the plasma membrane of neural cell bodies, but not axons or dendrites. Expression levels of the gangliosides that mAb 105/A2B5 binds to, including GT3 and its O-acetylated derivative, decrease during embryonic development of the brain. Thus, mAb 105/A2B5 may be used to monitor the maturation of brain tissue. A2B5 antigens have also been associated with numerous tumors, including glioblastoma multiforme tumor cells, pancreatic islet tumor cells, and rat insulinoma cells, and are thought to contribute to chemoresistance and increased tumor proliferation and recurrence. Flow cytometric analysis of A2B5 expression on mouse pancreatic tumor (insulinoma) cells and Human embryonic stem cell (hESC)-derived neurons. Mouse Beta-TC-6 cells (ATCC, CRL-11506, left panel) or human H9 (WiCell, Madison, WI) hESC-derived neurons (right panel) were harvested with Accutase™ Cell Detachment Solution (Cat. No. 561527). The cells were stained with either Purified Mouse IgM, κ Isotype Control (Cat. No. 555581, dashed line histograms) or Purified Mouse Anti- A2B5 monoclonal antibody (solid line histograms) at matched concentrations. Dot plots were derived from gated events based on light scattering characteristics of viable cells. Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-A2B5 - Purified - Clone 105/A2B5 (also known as antibody A2B5, F12A2B5, 105) - Isotype Mouse IgM - Reactivity Ms, Hu, Chick, Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Chicken embryo retina Cells

Clone: 105-A2B5

Isotype: IgM

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Référence

Balik, Mirossay, Bohus, Sulla, Mirossay, Sarissky: "Flow cytometry analysis of neural differentiation markers expression in human glioblastomas may predict their response to chemotherapy." dans: Cellular and molecular neurobiology, Vol. 29, Issue 6-7, pp. 845-58, (2009) (PubMed).

Eisenbarth, Walsh, Nirenberg: "Monoclonal antibody to a plasma membrane antigen of neurons." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, Issue 10, pp. 4913-7, (1980) (PubMed).

Détail du antigène

Antigène: A2B5