Androgen Receptor anticorps

N° du produit ABIN2688843

L’anticorps Souris Monoclonal anti-Androgen Receptor a été validé pour FACS, IP, IHC (f) et IHC (fro). Il convient pour détecter Androgen Receptor dans des échantillons de Humain et Rat. Il y a 2+ publications disponibles.

Aperçu rapide pour Androgen Receptor anticorps (ABIN2688843)

Antigène: Androgen Receptor (AR)

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Androgen Receptor est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: G122

Détail du produit anti-Androgen Receptor anticorps

Marque: BD Pharmingen™

Attributs du produit: The androgen receptor (AR) mediating androgen effects is a member of the steroid and thyroid hormone receptor gene superfamily encoding ligand-dependent nuclear transcription factors. They can be divided into three main domains, an N-terminal domain which modulates transcription efficiency, a central DNA domain which binds to a target gene hormone response element, and a C-terminal hormone binding domain. Androgens are necessary for normal male development and function. They exert their effects on target tissue through binding to the AR, followed by association of the AR complex with specific binding sites on DNA. This AR complex may induce or repress gene transcription. Mutations in the AR gene are associated with androgen insensitivity syndrome, a disorder that causes XY genotypic males to develop as phenotypic females because of their inability to respond to androgens. AR mutations have also been identified in human prostate cancer specimens and the LNCaP human prostate cancer cell line. Monoclonal and polyclonal antibodies to the AR have identified AR-positive cells in a variety of tissues including male and female sexual organs, kidney, liver, adrenal cortex, pituitary gland, skeletal, cardiac, and smooth muscle cells. The androgen receptor migrates at a molecular weight of ~100 kD in SDS-PAGE. G122-25 recognizes an epitope localized within the DNA-binding domain (between amino acids 486 and 651) of the human androgen receptor. Additionally, G122-25 has been shown to recognize androgen receptors in frozen rat prostate tissue sections. G122-25 does not cross-react with estrogen or progesterone receptors. Purified recombinant human androgen receptor protein was used as immunogen.

BD Pharmingen™ Purified Mouse Anti-Human Androgen Receptor - Purified - Clone G122-25 - Isotype Mouse IgG1 - Reactivity Hu, Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Purified human androgen receptor recombinant protein

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Androgen Receptor (ABIN2688843)

Fuller: "The steroid receptor superfamily: mechanisms of diversity." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 5, Issue 15, pp. 3092-9, (1992) (PubMed).

Newmark, Hardy, Tonb, Carter, Epstein, Isaacs, Brown, Barrack: "Androgen receptor gene mutations in human prostate cancer." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6319-23, (1992) (PubMed).

Détails sur Androgen Receptor

Antigène: Androgen Receptor (AR)

Autre désignation: Androgen Receptor

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding