Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

PRDM1 anticorps

PRDM1 Reactivité: Humain, Souris WB, IHC (p), ICS Hôte: Rat Monoclonal 6D3 unconjugated
N° du produit ABIN2688853
  • Antigène Voir toutes PRDM1 Anticorps
    PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))
    Reactivité
    • 62
    • 18
    • 12
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain, Souris
    Hôte
    • 41
    • 23
    • 3
    Rat
    Clonalité
    • 46
    • 21
    Monoclonal
    Conjugué
    • 45
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp PRDM1 est non-conjugé
    Application
    • 53
    • 35
    • 17
    • 11
    • 7
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Intracellular Staining (ICS)
    Marque
    BD Pharmingen™
    Attributs du produit
    The 6D3 monoclonal antibody specifically binds to mouse B lymphocyte-induced maturation protein 1 (Blimp-1) and crossreacts with human BLIMP-1. Blimp-1 is a 98 kDa zinc finger-containing protein that is encoded by the Prdm1 gene. Blimp-1 was first discovered as a transcriptional repressor of the IFN-β promoter. Blimp-1 is critical for primordial germ cells specification in the early embryo and induction of terminal cell differentiation in multiple somatic cell types. It is largely known as a master regulator of antibody-secreting plasma cells due to its function in the repression of PAX5, CIITA and cMYC genes. Blimp-1 also controls T cell homeostasis and effector differentiation through different mechanisms, including repression of IL-17 producing cells. Blimp-1 also plays roles in the maturation and activity of dendritic cells and natural killer cells. Analyses of Blimp-1 Expression Panel 1. Western blot analysis of Blimp-1 expression in human myeloma cell lines. Lysates from RPMI-8226 (ATCC CLL-155, Lane 1) and U266 (ATCC TIB-196, Lane 2) cells were electrophoresed, blotted and probed with Purified Rat Anti-Blimp-1 antibody (Cat. No. 564703) followed by HRP Goat Anti-Rat Ig (Cat. No. 554017), and ECL detection reagents. The antibody detects Blimp-1 isoforms of 88-92 kDa in both cell lysates and 76-78 kDa) in the U266 cell lysate. Note: an unspecific band (~250 kDa) may be observed upon overexposure of the membranes. Panel 2. Flow cytometric analysis of Blimp-1 expression in a human myeloma cell line. RPMI 8226 cells were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574/562725) and stained with either Purified Rat IgG2a, κ (Cat. No. 553927, dashed line histogram) or Purified Rat Anti-Blimp-1 antibody (solid line histogram) followed by staining with PE Goat Anti-Rat Ig (Cat. No. 560767). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System. Panel 3. Immunohistochemical staining of human Blimp-1 in human tonsils. Following antigen retrieval with BD Retrievagen A Buffer (Cat No. 550524), the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Rat IgG2a κ Isotype Control (Left Image) or Purified Rat Anti-Human Blimp-1 antibody (Right Image). A three-step staining procedure that employs Biotin Goat Anti-Rat Immunoglobulin (Cat. No. 559286), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. As shown in the Right Image, the Blimp-1 specific antibody primarily stained the nuclei of some Germinal Center cells. Original magnification: 40x.

    BD Pharmingen™ Purified Rat Anti-Blimp-1 - Purified - Clone 6D3 - Isotype Rat IgG2a, κ - Reactivity Hu, Ms - 0.1 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Mouse Blimp-1 Recombinant Protein
    Clone
    6D3
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product PRDM1 Anticorps primaire
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Chan, Chiang, Tsai, Su, Chen, Hou, Lin: "Absence of the transcriptional repressor Blimp-1 in hematopoietic lineages reveals its role in dendritic cell homeostatic development and function." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 11, pp. 7039-46, (2009) (PubMed).

    Johnston, Poholek, DiToro, Yusuf, Eto, Barnett, Dent, Craft, Crotty: "Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation." dans: Science (New York, N.Y.), Vol. 325, Issue 5943, pp. 1006-10, (2009) (PubMed).

    Jourdan, Caraux, De Vos, Fiol, Larroque, Cognot, Bret, Duperray, Hose, Klein: "An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization." dans: Blood, Vol. 114, Issue 25, pp. 5173-81, (2009) (PubMed).

    Martins, Cimmino, Liao, Magnusdottir, Calame: "Blimp-1 directly represses Il2 and the Il2 activator Fos, attenuating T cell proliferation and survival." dans: The Journal of experimental medicine, Vol. 205, Issue 9, pp. 1959-65, (2008) (PubMed).

    Ohinata, Payer, OCarroll, Ancelin, Ono, Sano, Barton, Obukhanych, Nussenzweig, Tarakhovsky, Saitou, Surani: "Blimp1 is a critical determinant of the germ cell lineage in mice." dans: Nature, Vol. 436, Issue 7048, pp. 207-13, (2005) (PubMed).

    Shaffer, Lin, Kuo, Yu, Hurt, Rosenwald, Giltnane, Yang, Zhao, Calame, Staudt: "Blimp-1 orchestrates plasma cell differentiation by extinguishing the mature B cell gene expression program." dans: Immunity, Vol. 17, Issue 1, pp. 51-62, (2002) (PubMed).

    Turner, Mack, Davis: "Blimp-1, a novel zinc finger-containing protein that can drive the maturation of B lymphocytes into immunoglobulin-secreting cells." dans: Cell, Vol. 77, Issue 2, pp. 297-306, (1994) (PubMed).

    Keller, Maniatis: "Identification and characterization of a novel repressor of beta-interferon gene expression." dans: Genes & development, Vol. 5, Issue 5, pp. 868-79, (1991) (PubMed).

  • Antigène
    PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))
    Autre désignation
    Blimp-1 (PRDM1 Produits)
    Synonymes
    anticorps GB11750, anticorps CG5249, anticorps Dmel\\CG5249, anticorps blimp-1, anticorps dBlimp-1, anticorps dBlimp1, anticorps Blimp-1, anticorps Blimp1, anticorps nrd, anticorps prdm1, anticorps ubo, anticorps wu:fb99c12, anticorps zgc:91885, anticorps MGC52536, anticorps PRDM1, anticorps blimp1, anticorps xblimp1, anticorps prdi-bf1, anticorps BLIMP1, anticorps PRDI-BF1, anticorps ZNFPR1A1, anticorps PR/SET domain 1, anticorps probable serine/threonine-protein kinase DDB_G0282963, anticorps CG5249 gene product from transcript CG5249-RF, anticorps blimp-1, anticorps PR domain containing 1a, with ZNF domain, anticorps PR domain 1 S homeolog, anticorps B lymphocyte induced maturation protein 1, anticorps PR domain 1, anticorps PR domain containing 1, with ZNF domain, anticorps PRDM1, anticorps LOC408295, anticorps Blimp-1, anticorps CpipJ_CPIJ007425, anticorps prdm1a, anticorps prdm1.S, anticorps prdm1, anticorps Prdm1
    Sujet
    Synonyms: BLIMP-1, PRDM1, PR domain containing 1, with ZNF domain, PRDI-BF1
    Pathways
    Regulation of Muscle Cell Differentiation
Vous êtes ici:
Support technique