Leukotriene B4 Receptor/BLT anticorps (Biotin)

N° du produit ABIN2688854

Cet anticorps Souris Monoclonal détecte spécifiquement Leukotriene B4 Receptor/BLT dans FACS. Il présente une réactivité avec des échantillons de Humain. Il a été cité dans 3+ publications.

Aperçu rapide pour Leukotriene B4 Receptor/BLT anticorps (Biotin) (ABIN2688854)

Antigène: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Leukotriene B4 Receptor/BLT est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: 203-14F11

Détail du produit anti-Leukotriene B4 Receptor/BLT anticorps (Biotin)

Attributs du produit: The monoclonal antibody 203/14F11 reacts against human leukotriene B4 receptor 1 (BLTR-1). BLTR-1 is a seven-transmembrane, G-protein-coupled receptor. The receptor plays an important role in pro-inflammatory responses. Leukotrienne B4 receptors are distributed in various hematopoietic cells including monocytes, granulocytes, and lymphocytes. Leukotriene B4 (LTB4), a product of arachidonic acid metabolism, is the ligand for BLTR-1. The immunogen used to generate 203/14F11 hybridoma was a human BLTR-1-transfected cell line. Detection of BLTR-1 expression on human peripheral monocytes and granulocytes by biotinylated 203/14F11. Human lysed whole blood were stained with 20 μL/test of biotinylated 203/14F11. Streptavidin-PE (Cat. No. 554061) was used as second-step reagent. The data reflects gating on monocytes (left panel) and granulocytes (right panel) based on forward and side scattered light signals. The level of nonspecific staining was assessed by using biotinylated mouse IgG1 (Cat. No. 555747) as isotype control (shaded histogram).

BD Pharmingen™ Biotin Mouse Anti-Human BLTR-1(Leukotriene B4 Receptor) - Biotin - Clone 203/14F11 - Isotype Mouse IgG1 - Reactivity Hu - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human BLTR-1-transfected cell line

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-Leukotriene B4 Receptor/BLT (Biotin) (ABIN2688854)

Pettersson, Boketoft, Sabirsh, Nilsson, Kotarsky, Olde, Owman: "First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1." dans: Biochemical and biophysical research communications, Vol. 279, Issue 2, pp. 520-5, (2001) (PubMed).

Dasari, Jin, Kunapuli: "Distribution of leukotriene B4 receptors in human hematopoietic cells." dans: Immunopharmacology, Vol. 48, Issue 2, pp. 157-63, (2000) (PubMed).

Yokomizo, Masuda, Kato, Toda, Izumi, Shimizu: "Leukotriene B4 receptor. Cloning and intracellular signaling." dans: American journal of respiratory and critical care medicine, Vol. 161, Issue 2 Pt 2, pp. S51-5, (2000) (PubMed).

Détails sur Leukotriene B4 Receptor/BLT

Antigène: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Autre désignation: BLTR-1Leukotriene B4 Receptor

Sujet: Synonyms: hBLTR-1