BMI1 anticorps

N° du produit ABIN2688857

Détail du produit anti-BMI1 anticorps

Antigène: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BMI1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), BioImaging (BI), Intracellular Staining (ICS)

Marque: BD Pharmingen™

Attributs du produit: The P51-311 monoclonal antibody binds to human BMI-1 (B lymphoma Mo-MLV insertion region 1 homolog). BMI1 is a c-myc cooperating oncogene that encodes an ~45 kDa protein that is a member of the Polycomb Group (PcG) of proteins. PcG proteins are essential for the maintenance, but not initiation, of the transcriptionally repressed state of certain developmental genes. PcG proteins are a structurally diverse group of proteins with conserved functions from fly to human cells. PcG proteins form complexes and regulate the expression of genes involved in cell cycle, DNA repair and differentiation that are crucial for maintaining the self renewal of normal and cancer stem cells. Specifically, BMI-1 is a core component of PRC1 (polycomb repressive complex 1). BMI-1, via the up-regulation of hTERT and independent of c-myc, can immortalize mammary epithelial cells. BMI-1 has also been shown to repress the INK4A locus that controls the tumor suppressors p16 and p19ARF (mouse homologue of p14ARF) in mouse models. BMI-1 plays a role in maintaining the self -renewal capacities of stem cells including hematopoietic, intestinal, retinal and neural stem cells. During antibody development, the purified P51-311 monoclonal antibody was found to detect BMI-1 by Western blot analysis of cellular lysates and by indirect immunofluorescent staining and flow cytometric analysis of fixed and permeabilized cells. Western blot analysis of BMI-1 expressed by human osteosarcoma cell line. Lysate from the human U-2 OS cell line (ATCC, HTB-96™) was electrophoresed (SDS-PAGE), transferred to a membrane and then probed with Purified Mouse Anti Human-BMI-1 antibody (Cat. No. 562527) at 4.0 μg/mL (lane 1), 2.0 μg/mL. (lane 2), and 1.0 μg/mL (lane 3). BMI-1 is identified as ~45 kDa band in the U-2 OS cell lysate by Western blotting. Image analysis of BMI-1 expressed in human osteosarcoma cell line. U-2 OS cells (ATCC, HTB-96™) were fixed with BD Cytofix™ Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) and stained with Purified Mouse Anti-Human-BMI-1 antibody (2.5 μg/mL, pseudo-colored green, Cat. No. 562527) followed by Alexa Fluor® 488 Goat Anti-Mouse Ig (Life Technologies, Cat. No. A-11001). BD Pharmingen™ Hoechst 33342 solution (Cat. No. 561908, pseudo-colored blue) was used for counterstaining. The image was captured using a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. BD™ Phosflow Perm/Wash Buffer I (Cat. No. 557885) and 0.1 % Triton™ X-100 (Sigma, Cat. No. X-100) are also suitable for permeabilization. 562527 Rev. 2 Page 1 of 2

BD Pharmingen™ Purified Mouse anti-Human BMI-1 - Purified - Clone P51-311 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human BMI-1 Recombinant Protein

Clone: P51

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-BMI1 (ABIN2688857)

Tian, Biehs, Warming, Leong, Rangell, Klein, de Sauvage: "A reserve stem cell population in small intestine renders Lgr5-positive cells dispensable." dans: Nature, Vol. 478, Issue 7368, pp. 255-9, (2011) (PubMed).

Sangiorgi, Capecchi: "Bmi1 is expressed in vivo in intestinal stem cells." dans: Nature genetics, Vol. 40, Issue 7, pp. 915-20, (2008) (PubMed).

Molofsky, Pardal, Iwashita, Park, Clarke, Morrison: "Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation." dans: Nature, Vol. 425, Issue 6961, pp. 962-7, (2003) (PubMed).

Park, Qian, Kiel, Becker, Pihalja, Weissman, Morrison, Clarke: "Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells." dans: Nature, Vol. 423, Issue 6937, pp. 302-5, (2003) (PubMed).

Dimri, Martinez, Jacobs, Keblusek, Itahana, Van Lohuizen, Campisi, Wazer, Band: "The Bmi-1 oncogene induces telomerase activity and immortalizes human mammary epithelial cells." dans: Cancer research, Vol. 62, Issue 16, pp. 4736-45, (2002) (PubMed).

Itahana, Zou, Itahana, Martinez, Beausejour, Jacobs, Van Lohuizen, Band, Campisi, Dimri: "Control of the replicative life span of human fibroblasts by p16 and the polycomb protein Bmi-1." dans: Molecular and cellular biology, Vol. 23, Issue 1, pp. 389-401, (2002) (PubMed).

Détails sur BMI1

Antigène: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Autre désignation: BMI-1 (BMI1 Produits)

Synonymes: anticorps FLVI2/BMI1, anticorps PCGF4, anticorps RNF51, anticorps AW546694, anticorps Bmi-1, anticorps Pcgf4, anticorps bmi1, anticorps pcgf4, anticorps psc1, anticorps wu:fb17g03, anticorps wu:fd18f06, anticorps BMI-1, anticorps pcgf4b, anticorps bmi-1, anticorps bmi1-a, anticorps bmi1-b, anticorps bmi1b, anticorps rnf51, anticorps xbmi-1, anticorps BMI1 proto-oncogene, polycomb ring finger, anticorps BMI1 polycomb ring finger oncogene, anticorps Bmi1 polycomb ring finger oncogene, anticorps bmi1 polycomb ring finger oncogene 1a, anticorps bmi1 polycomb ring finger oncogene 1b, anticorps BMI1 proto-oncogene, polycomb ring finger L homeolog, anticorps BMI1, anticorps LOC100230513, anticorps Bmi1, anticorps bmi1a, anticorps bmi1b, anticorps bmi1.L

Sujet: Synonyms: BMI1, PCGF4, polycomb group ring finger 4, RING finger protein 51, RNF51 Entrez Gene ID: 648

Poids moléculaire: ~45 kDa

Pathways: Cycle Cellulaire, Autophagy