BrdU anticorps

N° du produit ABIN2688858

L’anticorps Souris Monoclonal anti-BrdU a été validé pour IHC (fro), FM et ICS. Il convient pour détecter BrdU dans des échantillons de . Il y a 4+ publications disponibles.

Aperçu rapide pour BrdU anticorps (ABIN2688858)

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BrdU est non-conjugé

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Fluorescence Microscopy (FM), Intracellular Staining (ICS)

Clone: 3D4

Détail du produit anti-BrdU anticorps

Marque: BD Pharmingen™

Attributs du produit: Bromodeoxyuridine (BrdU) is an analog of thymidine that can be incorporated into newly synthesized DNA by cells entering and progressing thru the DNA synthesis (S) phase of the cell cycle. The amount of BrdU that gets incorporated is dependent upon the amount of time that the vells are exposed to BrdU (pulse time), the rate of cell division, and whether the cells are in early, mid, or late S phase. Detection of incorporated BrdU allows the investigator to identify cycling cells in an asynchronous cell population and to determine cell cycle kinetics. The 3D4 monoclonal antibody reacts with BrdU, but not other nucleotides, in single-stranded DNA. Random cleavage (nicking) of cellular DNA with DNase I permits the binding of the antibody to incorporated BrdU. Fluorescence Intensity (Linear) Profile of KG1A cells analyzed ona FACScan

BD Pharmingen™ Purified Mouse Anti- BrdU - Purified - Clone 3D4 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-BrdU (ABIN2688858)

Favero, Henshaw, Grimsley-Myers, Shrestha, Beier, Dwyer: "Mutation of the BiP/GRP78 gene causes axon outgrowth and fasciculation defects in the thalamocortical connections of the mammalian forebrain." dans: The Journal of comparative neurology, Vol. 521, Issue 3, pp. 677-96, (2013) (PubMed).

Allison, Barthel, Skebo, Takechi, Kawamura, Raymond: "Ontogeny of cone photoreceptor mosaics in zebrafish." dans: The Journal of comparative neurology, Vol. 518, Issue 20, pp. 4182-95, (2011) (PubMed).

Navailles, Hof, Schmauss: "Antidepressant drug-induced stimulation of mouse hippocampal neurogenesis is age-dependent and altered by early life stress." dans: The Journal of comparative neurology, Vol. 509, Issue 4, pp. 372-81, (2008) (PubMed).

Pecchi, Dallaporta, Charrier, Pio, Jean, Moyse, Troadec: "Glial fibrillary acidic protein (GFAP)-positive radial-like cells are present in the vicinity of proliferative progenitors in the nucleus tractus solitarius of adult rat." dans: The Journal of comparative neurology, Vol. 501, Issue 3, pp. 353-68, (2007) (PubMed).

Détails sur BrdU

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Autre désignation: BrdU

Classe de substances: Chemical