This antibody reacts with the 65-70 kDa common γ subunit (γc) shared by the IL-2, IL-4, IL-7, IL-9, and IL-15 receptors. The γc receptor is a glycoprotein expressed by most peripheral T and B lymphocytes, NK cells, monocytes, and granulocytes. The cytoplasmic domain of the γc chain plays an important role in cytokine-mediated signal transduction. By immunofluorescent staining and flow cytometric analysis, the TUGh4 antibody has been shown to specifically recognize human γc expressed by cell lines, including human γc gene-transfected cell lines, which are known to express the human γc chain.
BD Pharmingen™ Biotin Rat Anti-Human CD132 - Biotin - Clone TUGh4 - Isotype Rat IgG2b, κ - Reactivity Hu, Dog - 0.5 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
IL2RG
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C.
Ishii, Takeshita, Kimura, Tada, Kondo, Nakamura, Sugamura: "Expression of the IL-2 receptor gamma chain on various populations in human peripheral blood." dans: International immunology, Vol. 6, Issue 8, pp. 1273-7, (1995) (PubMed).
Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).