CTLA4 anticorps (Biotin)

N° du produit ABIN2688990

Il existe 4+ publications pour ce produit. L’anticorps anti-CTLA4 Monoclonal Souris est utilisé pour la détection de CTLA4 dans des échantillons de Humain. Il a été validé pour FACS.

Aperçu rapide pour CTLA4 anticorps (Biotin) (ABIN2688990)

Antigène: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CTLA4 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: BNI3

Détail du produit anti-CTLA4 anticorps (Biotin)

Attributs du produit: Reacts with the "cytolytic T lymphocyte-associated antigen", CTLA-4. CTLA-4 is transiently expressed on activated CD28+ T cells and binds to CD80 and CD86 present on antigen presenting cells (APC) with high avidity. This interaction appears to deliver a negative regulatory signal to the T cell. There are recent reports that indicate that CTLA-4 is also expressed on B cells when cultured with activated T cells, suggesting a possible role of CTLA-4 in the regulation of B-cell response. Immobilized BNI3.1 enhances T-cell proliferation induced by CD3 and CD28. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of Concanavalin A-stimulated peripheral blood mononuclear cells analyzed on a FACScan

BD Pharmingen™ Biotin Mouse Anti-Human CD152 - Biotin - Clone BNI3 - Isotype Mouse IgG2a, κ - Reactivity Hu - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CTLA4 (Biotin) (ABIN2688990)

Morton, Fu, Stewart, Giacoletto, White, Leysath, Evans, Shieh, Karr: "Differential effects of CTLA-4 substitutions on the binding of human CD80 (B7-1) and CD86 (B7-2)." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 3, pp. 1047-54, (1996) (PubMed).

Kuiper, Brouwer, Linsley, van Lier: "Activated T cells can induce high levels of CTLA-4 expression on B cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 4, pp. 1776-83, (1995) (PubMed).

Lindsten, Lee, Harris, Petryniak, Craighead, Reynolds, Lombard, Freeman, Nadler, Gray: "Characterization of CTLA-4 structure and expression on human T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3489-99, (1993) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Détails sur CTLA4

Antigène: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Autre désignation: CD152

Sujet: Synonyms: CTLA-4

Pathways: Cancer Immune Checkpoints