CD40 Ligand anticorps

N° du produit ABIN2688993

Détail du produit anti-CD40 Ligand anticorps

Antigène: CD40 Ligand (CD40LG)

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp CD40 Ligand est non-conjugé

Application: Flow Cytometry (FACS), Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marque: BD Pharmingen™

Attributs du produit: The MR1 antibody reacts with CD154 (CD40 Ligand, gp39), an accessory molecule expressed on activated T helper (CD4+) lymphocytes. CD154 has also been detected on other types of leukocytes, including CD8+ T cells, medullary thymocytes, activated CD4+ NK-T cells, and human NK cells. CD154 plays an important role in costimulatory interactions between T and B lymphocytes and between antigen-presenting cells and lymphocytes, regulating the immune response at multiple levels. MR1 mAb inhibits in vitro activation of B lymphocytes by T helper cells by blocking interaction of gp39 with CD40. In vitro interactions of T cells and antigen-presenting cells can also be blocked by the MR1 antibody. In vivo treatment with MR1 antibody blocks the development of experimental autoimmune disease, inhibits formation of germinal centers and generation of memory B cells, reduces T-lymphocyte responses to allogeneic cells and allografts, prevents intrathymic deletion of self-reactive T lymphocytes, and disrupts antigen-specific T-cell responses. CD154 expression on activated and resting T lymphocytes. BALB/c spleen T cells, purified on a T Cell Enrichment Column (R&D Systems), were cultured for 8 hours in the presence (Panels A and C) or absence (Panel B) of plate-bound 500A2 antibody (anti-CD3e, Cat. No. 553238). They were stained with purified MR1 antibody (Panels A and B), followed by biotinylated mouse anti-hamster IgG, Cat. No. 554010, then Streptavidin-PE, Cat. No. 554061, (Panels A, B, and C). Flow cytometry was performed on a BD FACScan™

BD Pharmingen™ Purified Hamster Anti-Mouse CD154 - Purified - Clone MR1 - Isotype Armenian Hamster IgG3, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Activated mouse Th1 clone D1.6

Clone: MR1

Isotype: IgG3 kappa

Information d'application

Indications d'application: Flow Cytometry System.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD40 Ligand (ABIN2688993)

Miga, Masters, Durell, Gonzalez, Jenkins, Maliszewski, Kikutani, Wade, Noelle: "Dendritic cell longevity and T cell persistence is controlled by CD154-CD40 interactions." dans: European journal of immunology, Vol. 31, Issue 3, pp. 959-65, (2001) (PubMed).

Graca, Honey, Adams, Cobbold, Waldmann: "Cutting edge: anti-CD154 therapeutic antibodies induce infectious transplantation tolerance." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 4783-6, (2000) (PubMed).

Lettesjö, Burd, Mageed: "CD4+ T lymphocytes with constitutive CD40 ligand in preautoimmune (NZB x NZW)F1 lupus-prone mice: phenotype and possible role in autoreactivity." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 4095-104, (2000) (PubMed).

Tomura, Yu, Ahn, Yamashita, Yang, Ono, Hamaoka, Kawano, Taniguchi, Koezuka, Fujiwara: "A novel function of Valpha14+CD4+NKT cells: stimulation of IL-12 production by antigen-presenting cells in the innate immune system." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 93-101, (1999) (PubMed).

Garside, Ingulli, Merica, Johnson, Noelle, Jenkins: "Visualization of specific B and T lymphocyte interactions in the lymph node." dans: Science (New York, N.Y.), Vol. 281, Issue 5373, pp. 96-9, (1998) (PubMed).

Grewal, Flavell: "CD40 and CD154 in cell-mediated immunity." dans: Annual review of immunology, Vol. 16, pp. 111-35, (1998) (PubMed).

Kalled, Cutler, Datta, Thomas: "Anti-CD40 ligand antibody treatment of SNF1 mice with established nephritis: preservation of kidney function." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 5, pp. 2158-65, (1998) (PubMed).

Carbone, Ruggiero, Terrazzano, Palomba, Manzo, Fontana, Spits, Kärre, Zappacosta: "A new mechanism of NK cell cytotoxicity activation: the CD40-CD40 ligand interaction." dans: The Journal of experimental medicine, Vol. 185, Issue 12, pp. 2053-60, (1997) (PubMed).

DeKruyff, Gieni, Umetsu: "Antigen-driven but not lipopolysaccharide-driven IL-12 production in macrophages requires triggering of CD40." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 1, pp. 359-66, (1997) (PubMed).

Dunn, Luedecker, Haugen, Clegg, Farr: "Thymic overexpression of CD40 ligand disrupts normal thymic epithelial organization." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 45, Issue 1, pp. 129-41, (1997) (PubMed).

Kawano, Cui, Koezuka, Toura, Kaneko, Motoki, Ueno, Nakagawa, Sato, Kondo, Koseki, Taniguchi: "CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides." dans: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1626-9, (1997) (PubMed).

Kelsall, Stüber, Neurath, Strober: "Interleukin-12 production by dendritic cells. The role of CD40-CD40L interactions in Th1 T-cell responses." dans: Annals of the New York Academy of Sciences, Vol. 795, pp. 116-26, (1997) (PubMed).

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." dans: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, (1997) (PubMed).

Griggs, Agersborg, Noelle, Ledbetter, Linsley, Tung: "The relative contribution of the CD28 and gp39 costimulatory pathways in the clonal expansion and pathogenic acquisition of self-reactive T cells." dans: The Journal of experimental medicine, Vol. 183, Issue 3, pp. 801-10, (1996) (PubMed).

Laman, Claassen, Noelle: "Functions of CD40 and its ligand, gp39 (CD40L)." dans: Critical reviews in immunology, Vol. 16, Issue 1, pp. 59-108, (1996) (PubMed).

Larsen, Elwood, Alexander, Ritchie, Hendrix, Tucker-Burden, Cho, Aruffo, Hollenbaugh, Linsley, Winn, Pearson: "Long-term acceptance of skin and cardiac allografts after blocking CD40 and CD28 pathways." dans: Nature, Vol. 381, Issue 6581, pp. 434-8, (1996) (PubMed).

Foy, Page, Waldschmidt, Schoneveld, Laman, Masters, Tygrett, Ledbetter, Aruffo, Claassen, Xu, Flavell, Oehen, Hedrick, Noelle: "An essential role for gp39, the ligand for CD40, in thymic selection." dans: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1377-88, (1995) (PubMed).

Roy, Aruffo, Ledbetter, Linsley, Kehry, Noelle: "Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses." dans: European journal of immunology, Vol. 25, Issue 2, pp. 596-603, (1995) (PubMed).

Tian, Noelle, Lawrence: "Activated T cells enhance nitric oxide production by murine splenic macrophages through gp39 and LFA-1." dans: European journal of immunology, Vol. 25, Issue 1, pp. 306-9, (1995) (PubMed).

Foy, Laman, Ledbetter, Aruffo, Claassen, Noelle: "gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory." dans: The Journal of experimental medicine, Vol. 180, Issue 1, pp. 157-63, (1994) (PubMed).

Détails sur CD40 Ligand

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD154 (CD40LG Produits)

Synonymes: anticorps CD154, anticorps cd40l, anticorps CD40L, anticorps HIGM1, anticorps IGM, anticorps IMD3, anticorps T-BAM, anticorps TNFSF5, anticorps TRAP, anticorps gp39, anticorps hCD40L, anticorps CD40-L, anticorps Cd40l, anticorps Ly-62, anticorps Ly62, anticorps Tnfsf5, anticorps CD40 ligand, anticorps TNF superfamily member 5, anticorps CD40LG, anticorps cd40l, anticorps Cd40lg

Sujet: Synonyms: CD40 Ligand, gp39

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints