CD16 anticorps

N° du produit ABIN2689003

Cet anticorps anti-CD16 est un anticorps Souris Monoclonal détectant CD16 dans FACS, IHC (fro), IHC (p) et IHC (zinc). Adapté pour Humain, Macaque rhésus, Cynomolgus et Babouin. Ce Primary Antibody a été cité dans 4+ publications.

Aperçu rapide pour CD16 anticorps (ABIN2689003)

Antigène: CD16

Reactivité: Humain, Macaque rhésus, Cynomolgus, Babouin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD16 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Clone: 3G8

Détail du produit anti-CD16 anticorps

Marque: BD Pharmingen™

Attributs du produit: The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (FcγRIII), a human NK cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes. The 3G8 antibody is reported (Vossebeld et al., 1997) to increase intracellular calcium levels in human neutrophils by interacting with both FcγRIIa and FcγRIIIb molecules. This antibody has also been reported to induce homotypic neutrophil aggregation. Immunohistochemical staining of human CD16 positive cells. Frozen sections of normal human spleen was stained with Purified Mouse Anti-Human CD16 (Cat. No. 550383) antibody. Cells positive for CD16 can be identified by intense brown labeling of their cell membranes. Amplification 20X.

BD Pharmingen™ Purified Mouse Anti-Human CD16 - Purified - Clone 3G8 - Isotype Mouse IgG1, κ - Reactivity Hu, Bab, Cyno, Rhe - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human polymorphonuclear leukocytes

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 125 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD16 (ABIN2689003)

Vossebeld, Homburg, Roos, Verhoeven: "The anti-Fc gamma RIII mAb 3G8 induces neutrophil activation via a cooperative actin of Fc gamma RIIIb and Fc gamma RIIa." dans: The international journal of biochemistry & cell biology, Vol. 29, Issue 3, pp. 465-73, (1997) (PubMed).

Wirthmueller, Kurosaki, Murakami, Ravetch: "Signal transduction by Fc gamma RIII (CD16) is mediated through the gamma chain." dans: The Journal of experimental medicine, Vol. 175, Issue 5, pp. 1381-90, (1992) (PubMed).

Stroncek, Skubitz, Plachta, Shankar, Clay, Herman, Fleit, McCullough: "Alloimmune neonatal neutropenia due to an antibody to the neutrophil Fc-gamma receptor III with maternal deficiency of CD16 antigen." dans: Blood, Vol. 77, Issue 7, pp. 1572-80, (1991) (PubMed).

Fleit, Wright, Unkeless: "Human neutrophil Fc gamma receptor distribution and structure." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 10, pp. 3275-9, (1982) (PubMed).

Détails sur CD16

Antigène: CD16

Autre désignation: CD16

Sujet: Synonyms: FcRIII, Fc-gamma RIII, FCG3, FCGR3, FCGRIII, FcγRIII, IGFR3

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process